Department of Materials Science and Engineering, African University of Science and Technology, Km 10 Airport Road, Galadimawa, Abuja, Federal Capital Territory (FCT), Nigeria; NASENI Centre of Excellence in Nanotechnology and Advanced Materials, Km 4, Ondo Road, Akure, Ondo State, Nigeria; Department of Mechanical and Materials Engineering, Worcester Polytechnic Institute (WPI), 100 Institute Road, Worcester, MA 01609, United States; Department of Biomedical Engineering, Worcester Polytechnic Institute, 60 Prescott Street, Gateway Park Life Sciences and Bioengineering Centre, Worcester, MA 01609, United States.
Department of Materials Science and Engineering, African University of Science and Technology, Km 10 Airport Road, Galadimawa, Abuja, Federal Capital Territory (FCT), Nigeria.
Acta Biomater. 2023 Mar 1;158:374-392. doi: 10.1016/j.actbio.2023.01.011. Epub 2023 Jan 12.
This paper presents the results of a combined experimental and theoretical study of the structure and viscoelastic properties of human non-tumorigenic mammary breast tissues and triple negative breast cancer (TNBC) tissues of different histological grades. A combination of immunofluorescence and confocal microscopy, and atomic force microscopy is used to study the actin cytoskeletal structures of non-tumorigenic and tumorigenic breast tissues (grade I to grade III). A combination of nanoindentation and statistical techniques is then used to measure viscoelastic properties of non-tumorigenic and human TNBC of different histological grades. A Standard Fluid Model/Anti-Zener Model II is also used to characterize the viscoelastic properties of the non-tumorigenic and tumorigenic TNBC tissues of different grades. The implications of the results are discussed for the potential application of nanoindentation and statistical deconvolution techniques to the development of mechanical biomarkers for TNBC detection/cancer diagnosis. STATEMENT OF SIGNIFICANCE: There is increasing interest in the development of mechanical biomarkers for cancer diagnosis. Here, we show that nanoindentation techniques can be used to characterize the viscoelastic properties of normal breast tissue and TNBC tissues of different histological grades. The Standard Fluid Model (Anti-Zener Model II) is used to classify the viscoelastic properties of breast tissues of different TNBC histological grades. Our results suggest that breast tissue and TNBC tissue viscoelastic properties can be used as mechanical biomarkers for the detection of TNBC at different stages.
本文介绍了一项综合实验和理论研究的结果，该研究旨在探讨人非致瘤性乳腺组织和不同组织学分级的三阴性乳腺癌（TNBC）组织的结构和黏弹性特性。采用免疫荧光和共聚焦显微镜以及原子力显微镜相结合的方法研究非致瘤性和致瘤性乳腺组织（I 级至 III 级）的肌动蛋白细胞骨架结构。然后，采用纳米压痕和统计技术相结合的方法测量非致瘤性和不同组织学分级的人 TNBC 的黏弹性特性。还采用标准流体模型/反泽纳模型 II 来表征不同分级的非致瘤性和致瘤性 TNBC 组织的黏弹性特性。讨论了这些结果对纳米压痕和统计去卷积技术在开发用于 TNBC 检测/癌症诊断的机械生物标志物方面的潜在应用的意义。 意义声明：人们越来越关注开发用于癌症诊断的机械生物标志物。在这里，我们表明，纳米压痕技术可用于表征正常乳腺组织和不同组织学分级的 TNBC 组织的黏弹性特性。采用标准流体模型（反泽纳模型 II）对不同 TNBC 组织学分级的乳腺组织的黏弹性特性进行分类。我们的结果表明，乳腺组织和 TNBC 组织的黏弹性特性可用作不同阶段 TNBC 检测的机械生物标志物。
