Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, Jiangsu Key Laboratory of Bio-functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, PR China.
Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, Jiangsu Key Laboratory of Bio-functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, PR China.
J Hazard Mater. 2021 Jun 5;411:125110. doi: 10.1016/j.jhazmat.2021.125110. Epub 2021 Jan 14.
Bacterial associated infection is a remaining urgent challenge in clinic application of metallic implants and devices. Here, we developed a new strategy to combat the bacterial associated infection of titanium alloy (TC4). Novel phosphonate/active ester block polymers (pDEMMP-b-pNHSMA) with identical phosphonate segments (DP = 29) as the metal anchorable ligand but varied active ester segments (DPs = 7, 29, and 64) as the conjugation site for poly(hexamethylene biguanide) (PHMB) were precisely prepared. Through a facile two-step process, the polymeric coating were successfully constructed on TC4 substrates as evidenced by water contact angle and XPS measurements. Through systematical in vitro antibacterial evaluations, robust relationship between the chemical structure of coating polymer and the antibacterial property endowed to the TC4 substrates has been established. Results showed that the block polymer, bearing an active ester segment of 64 repeat units, enabled dense packing of PHMB coating on the TC4 surface, which is able to kill 100% of both S. aureus and E. coli. that seeded without compromising the cytocompatibility of TC4 substrates. Furthermore, PHMB coating could significantly inhibit the colony of the bacteria and consequently reduce the bacterial associated inflammatory reaction as verified by a subcutaneous infection model on rat.
细菌相关感染是金属植入物和器械临床应用中仍然存在的一个紧迫挑战。在这里，我们开发了一种新策略来对抗钛合金（TC4）的细菌相关感染。新型膦酸酯/活性酯嵌段聚合物（pDEMMP-b-pNHSMA）具有相同的膦酸酯段（DP = 29）作为金属锚定配体，但不同的活性酯段（DPs = 7、29 和 64）作为聚六亚甲基双胍（PHMB）的连接位点被精确制备。通过简单的两步法，在 TC4 基底上成功构建了聚合物涂层，这可以通过水接触角和 XPS 测量来证明。通过系统的体外抗菌评价，已经建立了涂层聚合物的化学结构与赋予 TC4 基底的抗菌性能之间的稳健关系。结果表明，具有 64 个重复单元的活性酯段的嵌段聚合物能够使 PHMB 涂层在 TC4 表面紧密堆积，能够杀死 100%的金黄色葡萄球菌和大肠杆菌，而不会损害 TC4 基底的细胞相容性。此外，PHMB 涂层可以显著抑制细菌的菌落，从而减少细菌相关的炎症反应，这在大鼠皮下感染模型中得到了验证。
