Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, Institute of Insect Science and Technology, School of Life Sciences, South China Normal University, Guangzhou 510631, China.
Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, Illinois 60637, USA.
Genome Res. 2021 Jun;31(6):947-957. doi: 10.1101/gr.272153.120. Epub 2021 Apr 15.
Transfer RNAs (tRNAs) are essential for translation, and tRNA expression and modifications are regulated by many factors. However, the interplay between the microbiome and host tRNA profiles through host-microbiome interactions has not been explored. In this study, we investigated host-microbiome interactions via the tRNA profiling of four tissue types from germ-free and specific pathogen-free mice. Our analyses reveal that cytosolic and mitochondrial tRNA expression and tRNA modifications in the host are reprogrammed in a tissue-specific and microbiome-dependent manner. In terms of tRNA expression, the intestines and brains are more sensitive to the influence of the microbiome than the livers and kidneys. In terms of tRNA modifications, cytosolic tRNAs show more obvious changes in the livers and kidneys in the presence of the microbiome. Our findings reveal a previously unexplored relationship among the microbiome, tRNA abundance, and epitranscriptome in a mammalian host.
转移 RNA（tRNA）对于翻译至关重要，并且 tRNA 的表达和修饰受到许多因素的调节。然而，通过宿主-微生物组相互作用，微生物组与宿主 tRNA 谱之间的相互作用尚未得到探索。在这项研究中，我们通过无菌和特定病原体小鼠的四种组织类型的 tRNA 谱分析，研究了宿主-微生物组的相互作用。我们的分析表明，宿主中细胞质和线粒体 tRNA 的表达和 tRNA 修饰以组织特异性和微生物组依赖性的方式重新编程。就 tRNA 的表达而言，肠道和大脑比肝脏和肾脏更容易受到微生物组的影响。就 tRNA 修饰而言，在存在微生物组的情况下，肝脏和肾脏中的细胞质 tRNA 显示出更明显的变化。我们的研究结果揭示了微生物组、tRNA 丰度和哺乳动物宿主中的表观转录组之间以前未知的关系。
