Chen Ling-Yun, Qi Jing, Xu Hong-Lei, Lin Xiang-Yun, Sun Ya-Jun, Ju Shao-Qing
Center of Laboratory Medicine, Nantong Mental Health Center, Nantong, China.
Reaserch Center of Clinical Medicine, Affiliated Hospital of Nantong University, Nantong, China.
Front Psychiatry. 2021 Mar 30;12:637789. doi: 10.3389/fpsyt.2021.637789. eCollection 2021.
Schizophrenia is a severe mental disorder, which has a major impact on the quality of life and imposes a huge burden on the family. However, the pathogenesis of schizophrenia remains unclear and there are no specific biomarkers. Therefore, we intend to explore whether cf-DNA levels are related to the occurrence and development of schizophrenia. We analyzed and compared the concentration of cf-DNA in 174 SZ patients and 100 matched healthy controls by using quantitative real-time PCR by amplifying the repeats. We found that cf-DNA levels in peripheral blood reliably distinguished SZ patients from healthy controls ( < 0.05). The ROC analysis also supports the above conclusion. By tracking the absolute concentration of serum cf-DNA in primary cases, we found a distinct increase before treatment with antipsychotics, which decreased progressively after treatment. The present work indicates that cf-DNA may improve the efficiency of disease diagnosis, and the level of cf-DNA plays a predictive role in the development of schizophrenia. By evaluating the level of cf-DNA, we might play a certain role in a more reasonable and standardized clinical treatment of schizophrenia.
精神分裂症是一种严重的精神障碍，对生活质量有重大影响，并给家庭带来巨大负担。然而，精神分裂症的发病机制仍不清楚，且没有特异性生物标志物。因此，我们打算探讨游离DNA（cf-DNA）水平是否与精神分裂症的发生和发展有关。我们通过使用定量实时聚合酶链反应（PCR）扩增重复序列，分析并比较了174例精神分裂症患者和100例匹配的健康对照者的cf-DNA浓度。我们发现外周血中的cf-DNA水平能够可靠地将精神分裂症患者与健康对照者区分开来（P<0.05）。ROC分析也支持上述结论。通过追踪初发病例血清cf-DNA的绝对浓度，我们发现在使用抗精神病药物治疗前其明显升高，治疗后逐渐下降。目前的研究表明，cf-DNA可能提高疾病诊断效率，且cf-DNA水平在精神分裂症的发展中起预测作用。通过评估cf-DNA水平，我们可能在精神分裂症更合理、规范的临床治疗中发挥一定作用。
