Asfaw Geresu Minda, Assefa Wayuo Behailu, Mamo Kassa Gezahegne
Department of Veterinary Science, College of Agriculture and Environmental Science, Arsi University, Asella, Ethiopia.
College of Veterinary Medicine and Agriculture, Addis Ababa University, Bishoftu, Ethiopia.
Int J Microbiol. 2021 Mar 31;2021:6633522. doi: 10.1155/2021/6633522. eCollection 2021.
The status of and its antimicrobial susceptibility profile in animal origin food items from different catering establishments in Ethiopia is scarce. Hence, this study aimed to investigate the occurrence and antimicrobial susceptibility profile of isolates from animal origin food items in the selected areas of Arsi Zone. One hundred ninety-two animal origin food samples were collected and processed for isolation. Isolates were tested for their susceptibility to 13 antimicrobials using Kirby-Bauer disk diffusion assay. An overall prevalence of 9.4% (18/192) spp. isolates were recovered from animal origin food samples collected from different catering establishments. Seven (21.9%) of 4 (12.5%) of 3 (9.4%) of 2 (6.3%) of raw milk, 1 (3.1%) of egg sandwich and 1 (3.1%) of cream cake samples were positive for Catering establishments, protective clothing, source of contamination, manner of hand washing, and money handling were among the putative risk factors that were significantly associated ( < 0.05) with spp. occurrence. Ampicillin, nitrofurans, and sulphonamide resistance were significantly associated ( < 0.05) with spp. occurrence in the selected food items. Three (16.7%), 5 (27.8%), 5 (27.8%), and 4 (22.2%) of the isolates were resistant to 3, 4, 5, and 6 antibiotics, respectively, whereas only a sole isolate was resistant to two antibiotics (viz. ampicillin and kanamycin). In conclusion, the general sanitary condition of the catering establishments, utensils used, and personnel hygienic practices were not to the recommended standards in the current study. Besides, detection of multidrug-resistant strains of in animal origin food items from different catering establishments suggests the need for detailed epidemiological and molecular characterization of the pathogen so as to establish the sources of acquisition of resistant strains. Hence, implementation of prevention and control strategies from farm production to consumption of animal origin food items are crucial.
埃塞俄比亚不同餐饮场所动物源性食品中[具体细菌名称未给出]的状况及其抗菌药敏谱鲜有报道。因此，本研究旨在调查阿尔西地区选定区域动物源性食品中[具体细菌名称未给出]分离株的发生情况及其抗菌药敏谱。收集了192份动物源性食品样本并进行处理以分离[具体细菌名称未给出]。使用 Kirby-Bauer 纸片扩散法检测分离株对13种抗菌药物的敏感性。从不同餐饮场所收集的动物源性食品样本中，[具体细菌名称未给出] spp. 分离株的总体患病率为9.4%（18/192）。生牛奶样本中有7份（21.9%）、[具体细菌名称未给出]样本中有4份（12.5%）、[具体细菌名称未给出]样本中有3份（9.4%）、[具体细菌名称未给出]样本中有2份（6.3%）、鸡蛋三明治样本中有1份（3.1%）以及奶油蛋糕样本中有1份（3.1%）的[具体细菌名称未给出]检测呈阳性。餐饮场所、防护服、污染源、洗手方式和金钱处理是与[具体细菌名称未给出] spp. 发生显著相关（P<0.05）的假定风险因素。氨苄青霉素、硝基呋喃和磺胺类药物耐药性与选定食品中[具体细菌名称未给出] spp. 的发生显著相关（P<0.05）。分别有3份（16.7%）、5份（27.8%）、5份（27.8%）和4份（22.2%）分离株对3种、4种、5种和6种抗生素耐药，而只有一株分离株对两种抗生素（即氨苄青霉素和卡那霉素）耐药。总之，在本研究中，餐饮场所的总体卫生状况、使用的器具和人员卫生习惯未达到推荐标准。此外，在不同餐饮场所的动物源性食品中检测到多重耐药的[具体细菌名称未给出]菌株表明需要对该病原体进行详细的流行病学和分子特征分析，以确定耐药[具体细菌名称未给出]菌株的获取来源。因此，从农场生产到动物源性食品消费实施[具体细菌名称未给出]预防和控制策略至关重要。
