暴露于Cd2+的大肠杆菌DNA中的单链断裂。
Single-strand breakage in DNA of Escherichia coli exposed to Cd2+.
作者信息
Mitra R S, Bernstein I A
出版信息
J Bacteriol. 1978 Jan;133(1):75-80. doi: 10.1128/jb.133.1.75-80.1978.
Abstract
When a growing culture of Escherichia coli was exposed to 3 X 10(-6) M Cd2+, 85 to 95% of the cells lost their ability to form colonies on agar plates. Loss of viability was accompanied by considerable single-strand breakage in the DNA, with no detectable increase in double-strand breaks. A direct correlation appeared to exist between the number of single-strand breaks and the concentrations of Cd2+ to which the cells were exposed. Exposure of DNA in vitro to a Cd2+ concentration of 3 X 10(-6) M or higher, followed by sedimentation in alkaline sucrose gradients, demonstrated no single-strand breaks. Cadmium-exposed cells recovered viability when incubated in Cd2+-free liquid medium containing 10 mM hydroxyurea. During the early period of recovery, there was a lag in the incorporation of labeled thymidine, but cellular DNA, at least in part, appeared to be repaired.
摘要
当生长中的大肠杆菌培养物暴露于3×10⁻⁶ M的Cd²⁺时，85%至95%的细胞失去了在琼脂平板上形成菌落的能力。活力丧失伴随着DNA中大量的单链断裂，双链断裂未检测到增加。单链断裂的数量与细胞暴露于其中的Cd²⁺浓度之间似乎存在直接相关性。体外将DNA暴露于3×10⁻⁶ M或更高浓度的Cd²⁺，然后在碱性蔗糖梯度中沉降，未显示单链断裂。暴露于镉的细胞在含有10 mM羟基脲的无Cd²⁺液体培养基中孵育时恢复了活力。在恢复的早期，标记胸苷的掺入有一个延迟，但细胞DNA至少部分似乎得到了修复。
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