大肠杆菌在从低水平镉离子暴露中恢复过程中的蛋白质合成。
Protein synthesis in Escherichia coli during recovery from exposure to low levels of Cd2+.
作者信息
Mitra R S
出版信息
Appl Environ Microbiol. 1984 May;47(5):1012-6. doi: 10.1128/aem.47.5.1012-1016.1984.
Abstract
Exposure of Escherichia coli to 3 microM Cd2+ results in 84 to 95% of the cells losing their ability to form colonies on plates of nutrient agar. Transfer of the cells to Cd2+-free liquid medium results in a recovery of colony-forming ability without significant synthesis of DNA. As an early event in recovery, the cells exhibit a rapid uptake of [3H]leucine. Recovery and this incorporation are inhibited by chloramphenicol or rifampin. Sodium dodecyl sulfate-gel electrophoresis of proteins from recovering cells labeled with [3H]leucine for 1 min indicated the synthesis of at least two classes of proteins with apparent molecular weights of 55,000 to 65,000. One class bound Cd2+ and was absent in untreated cultures. The other class of proteins, which did not bind Cd2+, was synthesized at a rapid rate in recovering cells and may be a normal cellular protein.
摘要
将大肠杆菌暴露于3微摩尔的Cd2+中会导致84%至95%的细胞失去在营养琼脂平板上形成菌落的能力。将这些细胞转移至不含Cd2+的液体培养基中，菌落形成能力得以恢复，且DNA无显著合成。作为恢复过程中的早期事件，细胞表现出对[3H]亮氨酸的快速摄取。氯霉素或利福平会抑制恢复及这种掺入。对用[3H]亮氨酸标记1分钟的恢复细胞的蛋白质进行十二烷基硫酸钠-凝胶电泳，结果表明至少合成了两类表观分子量为55,000至65,000的蛋白质。一类结合Cd2+，在未处理的培养物中不存在。另一类不结合Cd2+的蛋白质在恢复细胞中快速合成，可能是一种正常的细胞蛋白质。
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