Nordin Norhanizam, Sani Nani Izreen Mohd, Kadir Arifah Abdul, Shaari Rumaizi, Mohamed Maizan, Reduan Mohd Farhan Hanif, Nordin Muhammad Luqman
Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, Pengkalan Chepa, 16100 Kota Bharu, Kelantan, Malaysia.
Faculty of Veterinary Medicine, Universiti Putra Malaysia (UPM), 43400 Serdang, Selangor, Malaysia.
J Adv Vet Anim Res. 2021 Mar 5;8(1):101-104. doi: 10.5455/javar.2021.h491. eCollection 2021 Mar.
In this case report, we have investigated the infectious bronchitis (IB) virus (IBV) outbreak with the co-infection of in 28-33-day-old broiler chickens in Malaysia.
A farmer complained that Cobb 500 chickens, raised in the open house, were having bloody diarrhea, open mouth breathing, non-uniform growth, and ruffled feathers. The mortality was about 100 birds (from about 7000 birds) per day. The sick birds were isolated and subjected to physical examination, postmortem, and histopathological analyses. Gross lesions were observed and recorded. The lung samples have proceeded with histopathological evaluations. The lungs, kidneys, trachea, air sac, and heart samples were collected to isolate bacteria and fungi through a series of conventional cultural methods, followed by molecular confirmation of the IBV.
Postmortem examination revealed air sacculitis, hemorrhagic tracheitis, pulmonary congestion, fibrin deposition in the liver and air sac, hemorrhagic enteritis, and renomegaly. The bacterial culture and biochemical tests revealed in the lungs, trachea, liver, intestine, and kidney samples. However, no fungus could be isolated from those samples. Histological evaluation of lung samples demonstrated infiltration of inflammatory cells in the pulmonary tissues. Apart from this, reverse transcription-polymerase chain reaction confirmed the presence of avian coronavirus responsible for infectious bronchitis (IB).
The chickens were diagnosed with IB concurrent with . The chickens exhibited typical nephropathogenic strain of IBV infection, causing high mortality.
在本病例报告中，我们调查了马来西亚28至33日龄肉鸡中传染性支气管炎（IB）病毒（IBV）与[此处原文缺失某种病原体名称]共同感染的疫情。
一位养殖户抱怨说，在开放式鸡舍饲养的科宝500肉鸡出现血便、张口呼吸、生长不均和羽毛蓬乱的症状。每天的死亡率约为100只鸡（约7000只鸡中）。对病鸡进行隔离，并进行体格检查、尸检和组织病理学分析。观察并记录大体病变。对肺样本进行组织病理学评估。采集肺、肾、气管、气囊和心脏样本，通过一系列传统培养方法分离细菌和真菌，随后对IBV进行分子确认。
尸检显示气囊炎、出血性气管炎、肺充血、肝脏和气囊中的纤维蛋白沉积、出血性肠炎和肾肿大。细菌培养和生化试验在肺、气管、肝脏、肠道和肾脏样本中发现了[此处原文缺失某种病原体名称]。然而，从这些样本中未分离出真菌。肺样本的组织学评估显示肺组织中有炎性细胞浸润。除此之外，逆转录-聚合酶链反应证实存在导致传染性支气管炎的禽冠状病毒。
这些鸡被诊断为感染IB并伴有[此处原文缺失某种病原体名称]。这些鸡表现出典型的肾病变型IBV感染，导致高死亡率。
