Department of Orthopaedics, Laboratory for Experimental Orthopaedics, Justus Liebig University Giessen, Giessen, Germany.
Mathematical Institute, Justus Liebig University Giessen, Giessen, Germany.
PLoS One. 2021 Apr 16;16(4):e0250146. doi: 10.1371/journal.pone.0250146. eCollection 2021.
The current limitations in evaluating synovial fluid (SF) components in health and disease and between species are due in part to the lack of data on normal SF, because of low availability of SF from healthy articular joints. Our study aimed to quantify species-dependent differences in phospholipid (PL) profiles of normal knee SF obtained from equine and human donors. Knee SF was obtained during autopsy by arthrocentesis from 15 and 13 joint-healthy human and equine donors, respectively. PL species extracted from SF were quantitated by mass spectrometry whereas ELISA determined apolipoprotein (Apo) B-100. Wilcoxon's rank sum test with adjustment of scores for tied values was applied followed by Holm´s method to account for multiple testing. Six lipid classes with 89 PL species were quantified, namely phosphatidylcholine, lysophosphatidylcholine, sphingomyelin, phosphatidylethanolamine, plasmalogen, and ceramide. Importantly, equine SF contains about half of the PL content determined in human SF with some characteristic changes in PL composition. Nutritional habits, decreased apolipoprotein levels and altered enzymatic activities may have caused the observed different PL profiles. Our study provides comprehensive quantitative data on PL species levels in normal human and equine knee SF so that research in joint diseases and articular lubrication can be facilitated.
当前在评估健康和疾病状态下以及不同物种间滑液（SF）成分方面存在的局限性部分归因于缺乏正常 SF 的数据，因为从健康关节中获得 SF 的方法并不普及。本研究旨在量化从马和人供体中获得的正常膝关节 SF 中磷脂（PL）谱的物种依赖性差异。通过关节穿刺术从 15 名和 13 名关节健康的人及马供体中分别获得膝关节 SF。通过质谱法定量 SF 中 PL 种类，而 ELISA 则测定载脂蛋白（Apo）B-100。应用经调整并列值得分的 Wilcoxon 秩和检验，然后使用 Holm 法进行多重检验校正。定量了 6 个脂质类别的 89 种 PL 种类，即磷脂酰胆碱、溶血磷脂酰胆碱、鞘磷脂、磷脂酰乙醇胺、血浆类脂和神经酰胺。重要的是，马 SF 中的 PL 含量约为人类 SF 中的一半，且 PL 组成存在一些特征性变化。营养习惯、载脂蛋白水平降低和酶活性改变可能导致了观察到的不同 PL 谱。本研究提供了正常人类和马膝关节 SF 中 PL 种类水平的综合定量数据，从而有助于关节疾病和关节润滑的研究。
