State Key Laboratory of Freshwater Ecology and Biotechnology, And Key Laboratory of Aquaculture Disease Control, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei Province, 430072, China; University of Chinese Academy of Sciences, Beijing, 100049, China; The Innovation Academy of Seed Design, Chinese Academy of Sciences, Wuhan, China.
State Key Laboratory of Freshwater Ecology and Biotechnology, And Key Laboratory of Aquaculture Disease Control, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei Province, 430072, China; The Innovation Academy of Seed Design, Chinese Academy of Sciences, Wuhan, China.
Dev Comp Immunol. 2021 Aug;121:104100. doi: 10.1016/j.dci.2021.104100. Epub 2021 Apr 19.
Toll-like receptors (TLRs), as a family of pattern recognition receptors (PRRs), possess specific pathogen-related molecular pattern (PAMP) recognition spectrum in inducing immune responses. In this study, sixteen TLRs were identified and characterized in mandarin fish (Siniperca chuatsi). All these TLRs consist of leucine-rich repeats (LRRs), a transmembrane domain and a Toll/interleukin-I receptor (TIR) domain, with the exception of TLR5S which lacks TIR domain, and they can be clustered into five branches, i.e. TLR1 subfamily, TLR3 subfamily, TLR5 subfamily, TLR7 subfamily and TLR11 subfamily in phylogenetic tree. These TLR genes were expressed in all tested tissues and had high expression levels in immune-related tissues such as head-kidney and spleen or mucosa-related tissues such as intestine and pyloric caecum. The transcripts of TLR2a, TLR2b, TLR3, TLR13a, TLR14, TLR22 and TLR23 were all significantly up-regulated after stimulation with poly(I:C); TLR1, TLR2a, TLR2b, TLR3, TLR5M, TLR5S, TLR13a and TLR13b transcripts were all significantly up-regulated after stimulation with PGN; and TLR2a, TLR2b, TLR5M, TLR5S, TLR7, TLR8, TLR9, TLR13c, TLR14 and TLR22 transcripts were all significantly up-regulated after stimulation with LPS in isolated head kidney lymphocytes of mandarin fish. The findings in this study may provide a valuable basis for functional study on TLR genes in mandarin fish.
Toll 样受体（TLRs）作为一类模式识别受体（PRRs），具有诱导免疫反应的特定病原体相关分子模式（PAMP）识别谱。在这项研究中，鉴定并描述了鳜鱼（Siniperca chuatsi）中的 16 种 TLR。所有这些 TLR 都包含富含亮氨酸的重复序列（LRRs）、跨膜结构域和 Toll/白细胞介素-I 受体（TIR）结构域，TLR5S 除外，它缺乏 TIR 结构域，它们可以在系统发生树上聚类为五个分支，即 TLR1 亚家族、TLR3 亚家族、TLR5 亚家族、TLR7 亚家族和 TLR11 亚家族。这些 TLR 基因在所有测试的组织中均有表达，在免疫相关组织（如头肾和脾脏）或黏膜相关组织（如肠和幽门盲囊）中表达水平较高。poly(I:C)刺激后，TLR2a、TLR2b、TLR3、TLR13a、TLR14、TLR22 和 TLR23 的转录本均显著上调；PGN 刺激后，TLR1、TLR2a、TLR2b、TLR3、TLR5M、TLR5S、TLR13a 和 TLR13b 的转录本均显著上调；LPS 刺激鳜鱼头肾淋巴细胞后，TLR2a、TLR2b、TLR5M、TLR5S、TLR7、TLR8、TLR9、TLR13c、TLR14 和 TLR22 的转录本均显著上调。本研究结果可为鳜鱼 TLR 基因的功能研究提供有价值的基础。
