Rifai N, Levtzow C B, Howlett C M, Phillips J C, Parker N C, Cross R E
Department of Pathology, University of North Carolina School of Medicine, Chapel Hill 27514.
J Anal Toxicol. 1988 May-Jun;12(3):150-2. doi: 10.1093/jat/12.3.150.
A method for the determination of the antidepressant drug trazodone is presented. 8-Hydroxyloxapine is used as the internal standard. A simple solid-phase extraction procedure utilizing disposable reversed-phase C18 columns is described. Samples are analyzed by gas chromatography with nitrogen-selective detection using a wide-bore capillary column with a permanently bonded, nonpolar stationary phase. The assay possesses linearity to 3.0 micrograms/mL, sensitivity to at least 0.25 microgram/mL, recovery averaging 96%, and between-run precision reflected by a CV of 5.6%. We conclude that the method reported here is ideally suited for monitoring therapeutic and toxic levels of trazodone.
本文介绍了一种测定抗抑郁药曲唑酮的方法。使用8-羟基奥氮平作为内标。描述了一种利用一次性反相C18柱的简单固相萃取程序。采用具有永久键合非极性固定相的宽口径毛细管柱，通过气相色谱和氮选择性检测对样品进行分析。该测定法线性范围达3.0微克/毫升，灵敏度至少为0.25微克/毫升，平均回收率为96%，批间精密度以5.6%的变异系数表示。我们得出结论，本文报道的方法非常适合监测曲唑酮的治疗和中毒水平。
