Department of Nanotechnology, SRM Institute of Science and Technology, Kattankulathur, Chengalpattu District, Tamil Nadu, 603 203, India.
Department of Nanotechnology, SRM Institute of Science and Technology, Kattankulathur, Chengalpattu District, Tamil Nadu, 603 203, India.
Photodiagnosis Photodyn Ther. 2021 Jun;34:102291. doi: 10.1016/j.pdpdt.2021.102291. Epub 2021 Apr 20.
Treating infection causing microorganisms is one of the major challenges in wound healing. These may gain resistance due to the overuse of conventional antibiotics. A promising technique is antimicrobial photodynamic therapy (aPDT) used to selectively cause damage to infectious pathogenic cells via generation of reactive oxygen species (ROS). We report on biocompatable nanomaterials that can serve as potential photosensitizers for aPDT. GO/Zn(Cu)O nanocomposite was synthesized by co-precipitation method. Graphene Oxide (GO) is known for its high surface to volume ratio, excellent surface functionality and enhanced antimicrobial property. ZnO nanoparticle induces the generation of reactive oxygen species (ROS) under light irradiation and it leads to recombination of electron-hole pair. Nanocomposites of GO and Cu doped ZnO increases visible light absorption and enhances the photocatalytic property. It generates more ROS and increases the bacterial inhibition. GO/Zn(Cu)O nanocomposite was tested against Staphylococcus aureus (S. aureus), Enterococcus faecium (E. faecium), Escherichia coli (E. coli), Salmonella typhi (S. typhi), Shigella flexneri (S. flexneri) and Pseudomonas aeruginosa (P. aeruginosa) by well diffusion method, growth curve, colony count, biofilm formation under both dark and visible light condition. Reactive Oxygen Species assay (ROS), Lactate dehydrogenase leakage (LDH) assay, Protein estimation assay and membrane integrity study proves the mechanism of inhibition of bacteria. Inhibition kinetics shows the sensitivity between bacteria and GO/Zn(Cu)O nanocomposite.
治疗感染致病微生物是伤口愈合的主要挑战之一。由于常规抗生素的过度使用，这些微生物可能会产生耐药性。一种有前途的技术是抗菌光动力疗法（aPDT），它用于通过生成活性氧物种（ROS）来选择性地对感染性致病细胞造成损伤。我们报告了可作为 aPDT 潜在光敏剂的生物相容性纳米材料。通过共沉淀法合成了 GO/Zn(Cu)O 纳米复合材料。氧化石墨烯（GO）以其高的比表面积、优异的表面功能和增强的抗菌性能而闻名。在光照射下，氧化锌纳米粒子诱导活性氧（ROS）的产生，导致电子-空穴对的复合。GO 和 Cu 掺杂 ZnO 的纳米复合材料增加了可见光吸收并增强了光催化性能。它产生更多的 ROS，并增加细菌抑制作用。通过平板扩散法、生长曲线、菌落计数、黑暗和可见光条件下的生物膜形成，对金黄色葡萄球菌（S. aureus）、屎肠球菌（E. faecium）、大肠杆菌（E. coli）、伤寒沙门氏菌（S. typhi）、福氏志贺菌（S. flexneri）和铜绿假单胞菌（P. aeruginosa）进行了 GO/Zn(Cu)O 纳米复合材料的测试。活性氧物种测定（ROS）、乳酸脱氢酶渗漏（LDH）测定、蛋白质估计测定和膜完整性研究证明了细菌抑制的机制。抑制动力学显示了细菌与 GO/Zn(Cu)O 纳米复合材料之间的敏感性。
