Department of Biochemistry, Bahauddin Zakariya University, Multan 60800, Pakistan.
Department of Biochemistry, Bahauddin Zakariya University, Multan 60800, Pakistan.
Bioelectrochemistry. 2021 Aug;140:107815. doi: 10.1016/j.bioelechem.2021.107815. Epub 2021 Apr 5.
Creatinine is an indicator of hindrance in urination and renal insufficiency. Creatinine levels are the marker of the late stages of prostate cancer. Early and sensitive detection of creatinine can reduce deaths associated with prostate cancer. In this work, nitrogen-doped porous carbon antimony (Sb/NPC) nanoparticles are fabricated to be employed as a non-enzymatic biosensor. Sb/NPC has promising redox activity and is synthesized by a two-step reaction using low-cost precursors. Electrochemical sensing by Sb/NPC is conducted for standard creatinine solutions on a three-electrodes system. Cyclic voltammetry, amperometry, and electrochemical impedance spectroscopy are used to sense creatinine. LOD and LOQ of the Sb/NPC modified electrode are 0.74 µM and 2.4 µM, respectively. This electrode system analyzes creatinine in the serum of prostate cancer patients who have elevated PSA levels. More than 90% creatinine is recovered from a spiked serum sample of a prostate cancer patient. A direct relation is observed between PSA levels and creatinine levels in prostate cancer. The developed cyclic voltammetric setup detects trace concentrations of creatinine in serum.
肌酐是排尿受阻和肾功能不全的一个指标。肌酐水平是前列腺癌晚期的标志物。早期、灵敏地检测肌酐可以降低与前列腺癌相关的死亡人数。在这项工作中，制备了掺氮多孔碳锑（Sb/NPC）纳米粒子作为非酶生物传感器。Sb/NPC 具有有前景的氧化还原活性，通过使用低成本前体制备的两步反应合成。通过三电极系统对标准肌酐溶液进行 Sb/NPC 的电化学传感。循环伏安法、安培法和电化学阻抗谱用于检测肌酐。Sb/NPC 修饰电极的 LOD 和 LOQ 分别为 0.74µM 和 2.4µM。该电极系统分析了 PSA 水平升高的前列腺癌患者血清中的肌酐。从前列腺癌患者的掺假血清样本中回收了超过 90%的肌酐。在前列腺癌中观察到 PSA 水平与肌酐水平之间存在直接关系。所开发的循环伏安装置检测血清中痕量浓度的肌酐。
