通过电极变形松弛进行单细胞力学分析和张力定量。
Single-cell mechanical analysis and tension quantification via electrodeformation relaxation.
机构信息
Department of Mechanical and Aerospace Engineering, Rutgers, The State University of New Jersey, 98 Brett Road, Piscataway, New Jersey 08854, USA.
Department of Biomedical Engineering, Rutgers, The State University of New Jersey, 599 Taylor Road, Piscataway, New Jersey 08854, USA.
出版信息
Phys Rev E. 2021 Mar;103(3-1):032409. doi: 10.1103/PhysRevE.103.032409.
The mechanical behavior and cortical tension of single cells are analyzed using electrodeformation relaxation. Four types of cells, namely, MCF-10A, MCF-7, MDA-MB-231, and GBM, are studied, with pulse durations ranging from 0.01 to 10 s. Mechanical response in the long-pulse regime is characterized by a power-law behavior, consistent with soft glassy rheology resulting from unbinding events within the cortex network. In the subsecond short-pulse regime, a single timescale well describes the process and indicates the naive tensioned (prestressed) state of the cortex with minimal force-induced alteration. A mathematical model is employed and the simple ellipsoidal geometry allows for use of an analytical solution to extract the cortical tension. At the shortest pulse of 0.01 s, tensions for all four cell types are on the order of 10^{-2} N/m.
采用电极弛豫法分析了单细胞的力学行为和皮质张力。研究了 MCF-10A、MCF-7、MDA-MB-231 和 GBM 这四种细胞，脉冲持续时间范围为 0.01 到 10 s。长脉冲状态下的力学响应表现出幂律行为，与皮层网络内的解联事件导致的软玻璃状流变一致。在亚秒级短脉冲状态下，单个时间尺度很好地描述了这个过程，并表明了皮层的原始张紧（预应力）状态，受到的力的影响最小。采用了一个数学模型，简单的椭球几何形状允许使用解析解来提取皮层张力。在最短的 0.01 s 脉冲下，所有四种细胞类型的张力都在 10^{-2} N/m 的量级。
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