Wanitschke R, Ammon H V
J Clin Invest. 1978 Jan;61(1):178-86. doi: 10.1172/JCI108916.
Perfusion studies of the normal human jejunum were performed to test whether dihydroxy bile acids and hydroxy fatty acids inhibit the absorption of oleic acid, since previous reports documented their inhibitory effects on the absorption of several other organic solutes. 3 mM deoxycholate and 7 mM glycodeoxycholate inhibited the absorption of 3 mM oleic acid in isotonic micellar solutions while inducing net fluid secretion. Similarly, fractional absorption of oleic acid decreased in the presence of hydroxy fatty acids. However, only the changes induced by 2 mM ricinoleic acid could be distinguished from changes induced by an increase in total fatty acid concentration. Under all experimental conditions, close linear relationships existed between net water movement and fractional absorption of glucose, xylose, and fatty acids, as well as between the absorption rates of these solutes. In contrast, net fluid secretion induced by hypertonic D-mannitol (450 mosmol/liter) had no effect on solute absorption. Our data and observations in the literature do not allow formulation of a hypothesis which would adequately define all effects of dihydroxy bile acids and fatty acids on intestinal transport processes. The observations help explain the malabsorption of fat and other nutrients in patients with the blind loop syndrome.
对正常人体空肠进行灌注研究，以测试二羟基胆汁酸和羟基脂肪酸是否会抑制油酸的吸收，因为先前的报告记录了它们对其他几种有机溶质吸收的抑制作用。3 mM脱氧胆酸盐和7 mM甘氨脱氧胆酸盐在等渗胶束溶液中抑制3 mM油酸的吸收，同时诱导净液体分泌。同样，在羟基脂肪酸存在的情况下，油酸的分数吸收降低。然而，只有2 mM蓖麻油酸引起的变化可以与总脂肪酸浓度增加引起的变化区分开来。在所有实验条件下，净水分移动与葡萄糖、木糖和脂肪酸的分数吸收之间，以及这些溶质的吸收速率之间都存在密切的线性关系。相比之下，高渗D-甘露醇（450 mosmol/升）诱导的净液体分泌对溶质吸收没有影响。我们的数据以及文献中的观察结果不允许形成一个能充分定义二羟基胆汁酸和脂肪酸对肠道转运过程所有影响的假设。这些观察结果有助于解释盲袢综合征患者脂肪和其他营养物质的吸收不良。
