Lamabadusuriya S P, Guiraldes E, Harries J T
Gastroenterology. 1975 Aug;69(2):463-9.
The influence of mixtures of taurocholate (TC), oleic acid (OA), caprylic acid (CA), and monolein (MO) on the toxic effects of deoxycholate (DC) in rat jejunum have been investigated using both a closed loop and perfusion technique. DC induced net secretion of water and electrolytes, inhibited glucose transport and transmural potential difference (PD), and inactivated mucosal "total" and (Na+ -K+)-adenosine triphosphatase. Secretion was reversed to absorption when the instilled or perfused solutions were composed of mixtures of DC, TC and OA; substitution of MO or CA for OA produced a similar effect. DC-induced inhibition of PD, glucose absorption, and mucosal adenosine triphosphatase activity was abolished when DC was mixed with TC and OA. Oleic acid emulsions had no effect on secretion induced by DC. Absorption of DC was inhibited from mixed micellar solutions (TC, OA, DC) but not from pure micellar solutions (TC, DC). These results indicate that the presence of taurocholate and fatty acids or monolein within the intestinal lumen markedly modify a number of the toxic effects of DC on jejunal function. The clinical effects of DC on intestinal function in man may therefore depend on the relative concentrations of other bile salts and lipids within the intestinal lumen.
使用闭环和灌注技术，研究了牛磺胆酸盐（TC）、油酸（OA）、辛酸（CA）和甘油单油酸酯（MO）的混合物对脱氧胆酸盐（DC）在大鼠空肠中毒性作用的影响。DC诱导水和电解质的净分泌，抑制葡萄糖转运和跨壁电位差（PD），并使黏膜“总”和（Na + -K +）-腺苷三磷酸酶失活。当灌注或滴注的溶液由DC、TC和OA的混合物组成时，分泌转变为吸收；用MO或CA替代OA产生类似效果。当DC与TC和OA混合时，DC诱导的PD抑制、葡萄糖吸收抑制和黏膜腺苷三磷酸酶活性被消除。油酸乳剂对DC诱导的分泌无影响。DC从混合胶束溶液（TC、OA、DC）中的吸收受到抑制，但从纯胶束溶液（TC、DC）中未受抑制。这些结果表明，肠腔内牛磺胆酸盐和脂肪酸或甘油单油酸酯的存在显著改变了DC对空肠功能的许多毒性作用。因此，DC对人体肠道功能的临床影响可能取决于肠腔内其他胆汁盐和脂质的相对浓度。
