Department of radiation oncology, QiLu hospital of ShanDong university, Jinan, Shandong 250012, PR China.
Department of radiation oncology, QiLu hospital of ShanDong university, Jinan, Shandong 250012, PR China.
Int J Biol Macromol. 2021 Jul 1;182:858-865. doi: 10.1016/j.ijbiomac.2021.04.082. Epub 2021 Apr 14.
G protein gamma 7 (GNG7) has been found to be aberrantly expressed in some kinds of malignant tumors. In this study, we mainly discuss the antitumor role of it in lung adenocarcinoma (LUAD) cells. Protein levels of GNG7 in LUAD tissues were measured by western blot and immunohistochemical analysis. Cell proliferation, invasion and migration were detected by CCK-8 assay, 5-ethynyl-2'-deoxyuridine (EdU), and Transwell assay. In our study, GNG7 was down-regulated in LUAD, which significantly correlated with survival of LUAD patients. Functional experiments revealed that GNG7 significantly inhibited LUAD cell proliferation, migration, and invasion in vitro and E2F1 overexpression reversed these properties. GNG7 suppressed xenograft tumorigenesis in nude mice models in vivo. In conclusion, GNG7 functions as a tumor suppressor in LUAD cells through inhibiting E2F1.
G 蛋白 γ 7（GNG7）在某些恶性肿瘤中表达异常。本研究主要探讨其在肺腺癌（LUAD）细胞中的抗肿瘤作用。通过 Western blot 和免疫组化分析检测 LUAD 组织中 GNG7 的蛋白水平。通过 CCK-8 测定、5-乙炔基-2'-脱氧尿苷（EdU）和 Transwell 测定检测细胞增殖、侵袭和迁移。在我们的研究中，GNG7 在 LUAD 中下调，与 LUAD 患者的生存显著相关。功能实验表明，GNG7 显著抑制 LUAD 细胞在体外的增殖、迁移和侵袭，而过表达 E2F1 逆转了这些特性。GNG7 在体内裸鼠模型中抑制异种移植肿瘤的发生。总之，GNG7 通过抑制 E2F1 在 LUAD 细胞中发挥肿瘤抑制作用。
