Wu Yang, Zhang Yuhan, Zhang Ren, Chen Shiyi
Department oft of Orthopaedic Sports Medicine, Huashan Hospital Affiliated to Fudan University, Shanghai, China.
Center for Analysis and Measurement, Fudan University, Shanghai, China.
Front Bioeng Biotechnol. 2021 Mar 31;9:630745. doi: 10.3389/fbioe.2021.630745. eCollection 2021.
Due to its great biomechanical property, the polyethylene terephthalate (PET) artificial ligament has become one of the most promising allografts for anterior cruciate ligament (ACL) reconstruction. However, because of its chemical and biological inertness, PET is not a favored scaffold material for osteoblast growth, which promotes the ligament-bone healing. Meanwhile, in consideration of prevention of potential infection, the prophylactic injection of antibiotic was used as a post-operative standard procedure but also has the increasing risk of bacterial resistance. To face these two contradictions, in this article we coated a polydopamine (PDA) nano-layer on the PET ligament and used the coating as the adhesion interlayer to introduce nano-hydroxyapatite (nHA) and silver atoms to the surface of PET ligament. Because of the mild self-polymerization reaction of dopamine, the thermogravity analysis (TGA), Raman spectrum, and tensile test results show that the modification procedure have no negative effects on the chemical stability and mechanical properties of the PET. The results of NIH3T3 cell culture show that the PDA and nHA could effectively improve the biocompatibility of PET artificial ligament for fibroblast growth, and staphylococcus aureus antibacterial test results show that the Ag atom provided an antibacterial effect for PET ligament. As shown in this paper, the nano-PDA coating modification procedure could not only preserve the advantages of PET but also introduce new performance characteristics to PET, which opens the door for further functionalization of PET artificial ligament for its advanced development and application.
由于其优异的生物力学性能，聚对苯二甲酸乙二酯（PET）人工韧带已成为前交叉韧带（ACL）重建中最具前景的同种异体移植物之一。然而，由于PET具有化学和生物惰性，它并非促进韧带-骨愈合的成骨细胞生长的理想支架材料。同时，考虑到预防潜在感染，预防性注射抗生素是术后的标准程序，但细菌耐药风险也在增加。为应对这两个矛盾，在本文中，我们在PET韧带上涂覆了一层聚多巴胺（PDA）纳米层，并将该涂层用作粘附中间层，以将纳米羟基磷灰石（nHA）和银原子引入PET韧带表面。由于多巴胺的温和自聚合反应，热重分析（TGA）、拉曼光谱和拉伸试验结果表明，改性过程对PET的化学稳定性和力学性能没有负面影响。NIH3T3细胞培养结果表明，PDA和nHA能有效提高PET人工韧带对成纤维细胞生长的生物相容性，金黄色葡萄球菌抗菌试验结果表明，Ag原子为PET韧带提供了抗菌作用。如本文所示，纳米PDA涂层改性过程不仅能保留PET的优点，还能为PET引入新的性能特征，为PET人工韧带的进一步功能化及其先进发展和应用打开了大门。
