Sadek Shimaa A, Hassanein Sarah S, Mohamed Ayman S, Soliman Amel M, Fahmy Sohair R
Physiology, Zoology Department, Faculty of Science, Cairo University, Giza, Egypt.
J Food Biochem. 2022 Mar;46(3):e13729. doi: 10.1111/jfbc.13729. Epub 2021 Apr 19.
The current study aimed to evaluate the antibacterial, anti-inflammatory, analgesic, and renoprotective effects of echinochrome pigment extracted from sea urchin. The disk diffusion method was used for the antibacterial activity of echinochrome against four different bacterial strains; Salmonella typhimurium, Pseudomonas aeroginosa, Staphylococcus aureus, and Listeria monocytogenes. While, acetic acid-induced writhing, formalin-induced licking, and hot plate latency assays evaluate the analgesic activity. The biochemical and oxidative stress markers of kidneys, as well as the histopathological examination, were measured to evaluate the renoprotective activity of echinochrome for cecal ligation and puncture-induced renal injury in rats. Echinochrome pigment exhibited in vitro antibacterial activity against all aforementioned bacterial species besides a powerful anti-inflammatory impact in vitro by the effective stabilization of the RBCs membrane and in vivo by decrease levels of serum IL6 and TNF-α. What's more, echinochrome showed a notable analgesic efficacy as well as an enhancement of the kidney's biochemical markers, oxidative stress status, and histopathological screening. Ech attenuated cecal ligation and puncture-induced renal injury by improving renal biomarkers, suppressing reactive oxygen species propagation as well as its antibacterial, anti-inflammatory, and anti-nociceptive activities. PRACTICAL APPLICATIONS: Sea urchins are rich in pharmacologically important quinone pigments, specifically echinochrome. The current study aimed to evaluate the role of echinochrome as a renal protective remedy in sepsis and clarify its biological activities. Echinochrome exhibited antibacterial activity in vitro against Salmonella typhimurium, Pseudomonas aeroginosa, Staphylococcus aureus, and Listeria monocytogenes. Our results revealed that echinochrome protects the kidney against damage caused by sepsis in rats. Echinochrome can use in the treatment of sepsis as an antibacterial, anti-inflammatory, and antioxidant agent.
本研究旨在评估从海胆中提取的海胆紫酮色素的抗菌、抗炎、镇痛和肾脏保护作用。采用纸片扩散法评估海胆紫酮对四种不同细菌菌株的抗菌活性，这四种菌株分别是鼠伤寒沙门氏菌、铜绿假单胞菌、金黄色葡萄球菌和单核细胞增生李斯特菌。同时，通过醋酸诱导扭体、福尔马林诱导舔足和热板潜伏期试验来评估镇痛活性。测量肾脏的生化和氧化应激标志物以及进行组织病理学检查，以评估海胆紫酮对盲肠结扎和穿刺诱导的大鼠肾损伤的肾脏保护活性。海胆紫酮色素除了通过有效稳定红细胞膜在体外具有强大的抗炎作用以及通过降低血清白细胞介素6和肿瘤坏死因子-α水平在体内具有抗炎作用外，还对上述所有细菌物种表现出体外抗菌活性。此外，海胆紫酮显示出显著的镇痛效果以及肾脏生化标志物、氧化应激状态和组织病理学筛查结果的改善。海胆紫酮通过改善肾脏生物标志物、抑制活性氧的传播以及其抗菌、抗炎和抗伤害感受活性，减轻了盲肠结扎和穿刺诱导的肾损伤。实际应用：海胆富含具有重要药理作用的醌类色素，特别是海胆紫酮。本研究旨在评估海胆紫酮在脓毒症中作为肾脏保护药物的作用，并阐明其生物学活性。海胆紫酮在体外对鼠伤寒沙门氏菌、铜绿假单胞菌、金黄色葡萄球菌和单核细胞增生李斯特菌具有抗菌活性。我们的结果表明，海胆紫酮可保护大鼠肾脏免受脓毒症引起的损伤。海胆紫酮可作为抗菌、抗炎和抗氧化剂用于脓毒症的治疗。
