细胞中一氧化氮的检测：历史技术与未来展望

Sensing Nitric Oxide in Cells: Historical Technologies and Future Outlook.

作者信息

Almeida Bethany, Rogers Katherine E, Nag Okhil K, Delehanty James B

机构信息

American Society for Engineering Education, Washington, D.C. 20036, United States.

Center for Bio/Molecular Science and Engineering, Code 6900, U.S. Naval Research Laboratory, Washington, D.C. 20375, United States.

出版信息

ACS Sens. 2021 May 28;6(5):1695-1703. doi: 10.1021/acssensors.1c00051. Epub 2021 Apr 19.

DOI:10.1021/acssensors.1c00051

PMID:33871990

Abstract

Nitric oxide (NO) is a critical cell signaling molecule with important roles in both normal cellular physiology and pathology. Over the past 20 years, multiple sensing modalities have been developed for the intracellular synthesis (endogenous) and release (exogenous) of NO. In this review, we survey the historical progression of NO sensing platforms, highlight the current state of the art, and offer a forward-looking view of how we expect the field of NO sensing to develop in the context of recent advances in bio-nanotechnology and nanoscale cellular biosensors.

摘要

一氧化氮（NO）是一种关键的细胞信号分子，在正常细胞生理和病理过程中均发挥着重要作用。在过去20年里，人们开发了多种用于检测细胞内一氧化氮合成（内源性）和释放（外源性）的传感方式。在本综述中，我们考察了一氧化氮传感平台的历史发展进程，突出了当前的技术水平，并基于生物纳米技术和纳米级细胞生物传感器的最新进展，对一氧化氮传感领域的未来发展方向提出前瞻性展望。