Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, 210029, China; Department of Dental Implantology, Affiliated Stomatological Hospital of Stomatology, Nanjing Medical University, Nanjing, 210029, China.
Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, 210029, China; Department of Dental Implantology, Affiliated Stomatological Hospital of Stomatology, Nanjing Medical University, Nanjing, 210029, China; Department of Stomatology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China.
Colloids Surf B Biointerfaces. 2021 Jul;203:111768. doi: 10.1016/j.colsurfb.2021.111768. Epub 2021 Apr 14.
In order to improve early osseointegration and long-term survival rate of implants, a multifunctional titanium surface that promotes osteogenesis and antibacterial properties is expected. Incorporation of bioactive trace elements such as magnesium ions was proved a promising method to improve osseointegration of titanium. Phytic acid has strong chelating ability with multivalent cations, which has been used in surface modification. Moreover, phytic acid was proved antibacterial potential. Herein, to improve the osteogenic and antibacterial properties, a phytic acid-magnesium (PA-Mg) layer was introduced on titanium using phytic acid as a cross-linker molecule. No obvious changes of the surface characterization were observed by scanning electron microscopy and atomic force microscopy. X-ray photoelectron spectroscopy confirmed that the PA-Mg layer covalently bond to the Ti surface, and the thickness of the PA-Mg layer was about 150 nm. Besides, improved hydrophilic and more protein adsorption were observed on Ti-PA-Mg. Notably, a relatively controlled magnesium release was also observed on Ti-PA-Mg. Human bone mesenchymal stem cells showed better adhesion, proliferation, and osteogenic differentiation on Ti-PA-Mg samples, indicating improved biocompatibility and osteoinductivity. Moreover, Ti-PA-Mg had better antibacterial properties against porphyromonas gingivalis than Ti. Overall, the PA-Mg layer on Ti surface improved the osteogenic and antibacterial properties, which may have promise for use in dental implantation.
为了提高种植体的早期骨整合和长期存活率，人们期望得到一种具有促进成骨和抗菌性能的多功能钛表面。研究证明，掺入镁离子等生物活性微量元素是提高钛骨整合的一种很有前途的方法。植酸具有与多价阳离子的强螯合能力，已被用于表面改性。此外，植酸具有抗菌潜力。在此，为了提高成骨和抗菌性能，本文使用植酸作为交联分子，在钛上引入植酸-镁（PA-Mg）层。扫描电子显微镜和原子力显微镜观察到表面特性没有明显变化。X 射线光电子能谱证实 PA-Mg 层与 Ti 表面形成共价键，PA-Mg 层的厚度约为 150nm。此外，Ti-PA-Mg 表现出更好的亲水性和更多的蛋白质吸附。值得注意的是，Ti-PA-Mg 也表现出相对可控的镁释放。人骨髓间充质干细胞在 Ti-PA-Mg 样品上表现出更好的粘附、增殖和成骨分化，表明其具有更好的生物相容性和成骨诱导性。此外，Ti-PA-Mg 对牙龈卟啉单胞菌的抗菌性能优于 Ti。总之，Ti 表面的 PA-Mg 层提高了成骨和抗菌性能，有望用于牙科植入物。
