Aalborg University, Department of Chemistry and Bioscience, Fredrik Bajers Vej 7H, 9220 Aalborg, Denmark.
Aalborg University, Department of Chemistry and Bioscience, Niels Bohrsvej 8, 6700 Esbjerg, Denmark.
Int J Food Microbiol. 2021 Jun 2;347:109171. doi: 10.1016/j.ijfoodmicro.2021.109171. Epub 2021 Mar 26.
Potatoes contain several nutrients essential for fungal growth, making them an excellent component of media such as the popular Potato Dextrose Agar (PDA) medium. Commercially, PDA is available from multiple retailers offering virtually the same product. These media, however, could contain small differences in composition of nutrients affecting the expression of secondary metabolites. This study aims to investigate the use of four PDA media from different manufacturers (Fluka, Oxoid, Sigma, and VWR) and their effect on the metabolite profile of four species of Fusarium (F. fujikuroi, F. graminearum, F. pseudograminearum and F. avenaceum). Secondary metabolites were analysed using HPLC-HRMS, from which statistically significant differences in intensities were observed for 9 out of 10 metabolites.
土豆含有几种真菌生长所必需的营养物质，使它们成为像常用的土豆葡萄糖琼脂（PDA）培养基这样的培养基的极好成分。在商业上，PDA 可从多个零售商处获得，这些零售商提供的产品几乎相同。然而，这些培养基在影响次生代谢物表达的营养成分组成上可能存在细微差异。本研究旨在调查使用来自不同制造商（Fluka、Oxoid、Sigma 和 VWR）的四种 PDA 培养基及其对四种镰刀菌（F. fujikuroi、F. graminearum、F. pseudograminearum 和 F. avenaceum）代谢物谱的影响。使用 HPLC-HRMS 分析次生代谢物，从 10 种代谢物中观察到 9 种代谢物的强度存在统计学显著差异。
