Hassan Yousef I, Bullerman Lloyd B
Food Science and Technology Department, University of Nebraska-Lincoln, 349 Food Industry Complex, Lincoln, Nebraska 68583-0919, USA.
J Food Prot. 2008 Nov;71(11):2213-6. doi: 10.4315/0362-028x-71.11.2213.
Lactobacillus paracasei subsp. tolerans, isolated from a traditional sourdough bread culture and previously shown to have antifungal activity against Fusarium species, was tested for inhibition of growth of Fusarium proliferatum M 5991 and M 5689 and F. graminearum R 4053 in a liquid medium setting. This isolate completely inhibited the growth of F. proliferatum M 5689 and M 5991 and F. graminearum R 4053, whereas such growth was not inhibited in the control in a supernatant agar plate assay. When this isolate was tested using 2M medium (MRS-modified Myro media) known for supporting Fusarium growth and trichothecene production, it was found to inhibit fungal growth but promote mycotoxin production at the same time. The antifungal activity was determined to be the result of organic acids and low pH. The mechanism of the mycotoxin production promotion requires further investigation.
从传统酸面团培养物中分离出的副干酪乳杆菌耐酸亚种，此前已证明其对镰刀菌属具有抗真菌活性，在液体培养基环境中测试了其对轮枝镰刀菌M 5991和M 5689以及禾谷镰刀菌R 4053生长的抑制作用。该分离株完全抑制了轮枝镰刀菌M 5689和M 5991以及禾谷镰刀菌R 4053的生长，而在对照的上清琼脂平板试验中，这种生长未受到抑制。当使用以支持镰刀菌生长和单端孢霉烯产生而闻名的2M培养基（改良的MRS - Myro培养基）对该分离株进行测试时，发现它抑制真菌生长，但同时促进霉菌毒素的产生。确定抗真菌活性是有机酸和低pH的结果。霉菌毒素产生促进的机制需要进一步研究。
