Du Rui, Bing Hui, Lu Wentian, Gao Congting, Kong Fanxue, Wang Hongtu, Shen Yue, Liu Chongxi, Xiang Wensheng
Key Laboratory of Agricultural Microbiology of Heilongjiang Province, Northeast Agricultural University, Harbin, 150030, China.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
Antonie Van Leeuwenhoek. 2025 Mar 25;118(4):62. doi: 10.1007/s10482-025-02073-0.
A novel endophytic actinomycete with antagonistic activity against Fusarium moniliforme, designated strain NEAU-BLH26, was isolated from the root of Adonis amurensis Regel collected at the horticultural experiment station of Northeast Agricultural University, Heilongjiang Province, northeast China. Strain NEAU-BLH26 exhibited morphological and chemotaxonomic features of the genus Streptomyces. The diamino acid present in its cell wall was identified as LL-diaminopimelic acid, while galactose detected in whole-cell hydrolysates. The menaquinones were identified as MK-9(H), MK-9(H), and MK-9(H). The phospholipid profile comprised diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, two unidentified phospholipids and two unidentified aminolipids. The major fatty acids were determined to be iso-C and C. The DNA G + C content, based on the genome sequence, was 70.5 mol%. Phylogenetic analyses of 16S rRNA gene and whole genome sequences analyses indicated that strain NEAU-BLH26 was most closely related to Streptomyces geranii A301, with a 16S rRNA gene sequence similarity of 98.69%. However, the average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values confirmed that strain NEAU-BLH26 represents a distinct species. This conclusion was further supported by phenotypic and chemotaxonomic differences between strain NEAU-BLH26 and its closest relative. Additionally, the secondary metabolite biosynthetic gene clusters in strain NEAU-BLH26 were predicted and analyzed. Based on phenotypic, chemotaxonomic and genotypic evidence, strain NEAU-BLH26 is proposed as a novel species of the genus Streptomyces, for which the name Streptomyces adonidis sp. nov. is suggested. The type strain is NEAU-BLH26 (= JCM 36414 = MCCC 1K08678).
从中国东北黑龙江省东北农业大学园艺实验站采集的冰凉花（Adonis amurensis Regel）根部分离出一株对串珠镰刀菌（Fusarium moniliforme）具有拮抗活性的新型内生放线菌，命名为菌株NEAU - BLH26。菌株NEAU - BLH26表现出链霉菌属的形态和化学分类学特征。其细胞壁中存在的二氨基酸被鉴定为LL - 二氨基庚二酸，而在全细胞水解产物中检测到半乳糖。甲基萘醌被鉴定为MK - 9(H)、MK - 9(H)和MK - 9(H)。磷脂谱包括双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰肌醇、两种未鉴定的磷脂和两种未鉴定的氨基脂质。主要脂肪酸被确定为异-C 和 C。基于基因组序列，DNA G + C 含量为 70.5 mol%。16S rRNA 基因的系统发育分析和全基因组序列分析表明，菌株 NEAU - BLH26 与香叶链霉菌（Streptomyces geranii）A301 关系最为密切，16S rRNA 基因序列相似性为 98.69%。然而，平均核苷酸同一性、平均氨基酸同一性和数字 DNA - DNA 杂交值证实菌株 NEAU - BLH26 代表一个独特的物种。菌株 NEAU - BLH26 与其最接近的亲缘种之间的表型和化学分类学差异进一步支持了这一结论。此外，对菌株 NEAU - BLH26 中的次生代谢物生物合成基因簇进行了预测和分析。基于表型、化学分类学和基因型证据，建议将菌株 NEAU - BLH26 作为链霉菌属的一个新物种，命名为 Streptomyces adonidis sp. nov.。模式菌株为 NEAU - BLH26（= JCM 36414 = MCCC 1K08678）。
