Peer Wendy Ann, Mamoudian Mehrzad, Lahner Brett, Reeves Roger D, Murphy Angus S, Salt David E
Center for Environmental Stress Physiology, Department of Horticulture, 625 Agriculture Mall Drive, Purdue University, West Lafayette, IN 47907 USA.
Institute of Fundamental Sciences-Chemistry, Massey University, Palmerston North 5301, New Zealand.
New Phytol. 2003 Aug;159(2):421-430. doi: 10.1046/j.1469-8137.2003.00822.x.
• Here we report on the first phase of a funded programme to select a wild relative of Arabidopsis thaliana for use in large-scale genomic strategies, including forward and reverse genetic screens for the identification of genes involved in metal hyperaccumulation. • Twenty accessions of metal accumulating species of the Brassicaceae collected from Austria, France, Turkey and the USA during spring-summer 2001 were evaluated. • The criteria established for selection were: hyperaccumulation of metal (Ni, Zn); compact growth habit; reasonable time to flowering; production of ≥ 1000 seeds per plant; self-fertility; a compact diploid genome; high sequence identity with A. thaliana; and ≥ 0.1% transformation efficiency with easy selection. As part of this selection process we also report, for the first time, the stable genetic transformation of various hyperaccumulator species with both the green fluorescence protein (GFP) and the bar selectable marker. • We conclude that metal hyperaccumulation ability, self-fertility, seed set, transformation efficiency and a diploid genome were the most important selection criteria. Based on an overall assessment of the performance of all 20 accessions, Thlaspi caerulescens Félix de Pallières showed the most promise as a model hyperaccumulator.
• 在此，我们报告一项资助项目第一阶段的情况，该项目旨在挑选拟南芥的一个野生近缘种，用于大规模基因组策略，包括正向和反向遗传筛选，以鉴定参与金属超积累的基因。
• 对2001年春夏季从奥地利、法国、土耳其和美国收集的十字花科金属积累物种的种质进行了评估。
• 确立的选择标准为：金属（镍、锌）超积累；紧凑的生长习性；合理的开花时间；单株种子产量≥1000粒；自交育性；紧凑的二倍体基因组；与拟南芥的高序列同一性；以及易于筛选且转化效率≥0.1%。作为该选择过程的一部分，我们还首次报告了用绿色荧光蛋白（GFP）和bar选择标记对各种超积累物种进行稳定遗传转化的情况。
• 我们得出结论，金属超积累能力、自交育性、结实率、转化效率和二倍体基因组是最重要的选择标准。基于对所有20种种质表现的全面评估，天蓝遏蓝菜费利克斯·德帕利埃变种显示出作为模式超积累植物的最大潜力。
