Smith Caroline M, Rodriguez-Buey Marisa, Karlsson Jan, Campbell Malcolm M
Department of Plant Sciences, University of Oxford, South Parks Road, Oxford OX1 3RB, UK.
Umeå Plant Science Centre, Department of Plant Physiology, Umeå University, SE-901 87 Umeå, Sweden.
New Phytol. 2004 Oct;164(1):123-136. doi: 10.1111/j.1469-8137.2004.01151.x.
• The Populus-Poplar Mosaic Virus (PopMV) pathosystem is the best characterized of all forest tree-virus interactions. The details of the host response to this virus are completely unknown. • The transcript abundance for approximately 10 000 Populus genes was simultaneously interrogated using spotted cDNA microarrays. Relative transcript abundance was compared for RNA extracted from Populus leaves that were untreated, mock-inoculated leaves that were wounded by leaf abrasion and inoculated leaves that were abraded and then infected by virus. • Statistical analysis of the microarray data identified suites of genes that exhibited increased or decreased transcript abundance in response to wounding, systemic PopMV infection or both together. Genes implicated in programmed cell death, and cell wall reinforcement were a major feature of the wound response, whereas genes encoding metallothionein-like proteins, and proteins implicated in cell wall remodelling were a major feature of the PopMV response. • The identification of wound- and PopMV-regulated genes opens the door for future studies aimed at testing specific hypotheses related to the mechanisms used by forest trees to contend with stress.
• 胡杨花叶病毒（PopMV）致病系统是所有林木与病毒相互作用中特征描述最为详尽的。宿主对这种病毒的反应细节完全未知。
• 使用斑点cDNA微阵列同时检测了约10000个胡杨基因的转录本丰度。比较了从未经处理的胡杨叶片、经叶片擦伤模拟接种的叶片以及经擦伤后感染病毒的接种叶片中提取的RNA的相对转录本丰度。
• 对微阵列数据的统计分析确定了几组基因，这些基因在受伤、系统性PopMV感染或两者共同作用下，转录本丰度呈现增加或减少。参与程序性细胞死亡和细胞壁强化的基因是伤口反应的主要特征，而编码类金属硫蛋白的基因以及参与细胞壁重塑的蛋白质是PopMV反应的主要特征。
• 伤口和PopMV调控基因的鉴定为未来旨在检验与林木应对胁迫机制相关的特定假设的研究打开了大门。
