Rong Jun, Xia Hui, Zhu Youyong, Wang Yunyue, Lu Bao-Rong
The Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, Institute of Biodiversity Science, Fudan University, Shanghai 200433, China.
Ministry of Education Key Laboratory of Agriculture Biodiversity for Plant Disease Management, Key Laboratory of Plant Pathology, Yunnan Agricultural University, Kunming 650201, China.
New Phytol. 2004 Aug;163(2):439-445. doi: 10.1111/j.1469-8137.2004.01100.x.
• Mixed-planting of traditional and hybrid rice (Oryza sativa) varieties is an ecological approach for rice disease control and yield increase, in addition to its effective role in in situ conservation of traditional rice varieties. To estimate gene flow between traditional and hybrid rice varieties, an experiment involving Huangkenuo and Shanyou-63 was conducted to allow free gene flow by mixed-planting of the two varieties in different cultivation patterns. • A simple sequence repeat (SSR) marker RM167 was used to detect natural hybrids between the two varieties by examining seeds collected randomly from both varieties in the experiment. • The result showed an extremely low number of hybrids between the two varieties with an average frequency of 0.04% in Huangkenuo and 0.18% in Shanyou-63. • It is concluded that no significant gene flow occurs naturally between Huangkenuo and Shanyou-63, and that crop diversity management is a proper means for in situ conservation of traditional rice varieties. It is also implied that the potential transgene flow from transgenic hybrid rice to traditional rice variety would be extremely low.
• 传统水稻品种与杂交水稻品种混种，除了在传统水稻品种原地保护方面发挥有效作用外，还是一种控制水稻病害和提高产量的生态方法。为了估计传统水稻品种与杂交水稻品种之间的基因流动，开展了一项涉及黄壳糯和汕优63的实验，通过以不同种植模式将这两个品种混种来实现自由基因流动。
• 利用简单序列重复（SSR）标记RM167，通过检查实验中从两个品种随机收集的种子，来检测两个品种之间的天然杂交种。
• 结果表明，两个品种之间的杂交种数量极少，黄壳糯中的平均频率为0.04%，汕优63中的平均频率为0.18%。
• 得出的结论是，黄壳糯和汕优63之间自然情况下不会发生显著的基因流动，作物多样性管理是传统水稻品种原地保护的一种适当手段。这也意味着从转基因杂交水稻到传统水稻品种的潜在转基因流动将极低。
