Dickson S
Centre for Soil-Plant Interactions, Soil and Land Systems (Waite Campus), School of Earth and Environmental Sciences, The University of Adelaide, Adelaide, South Australia 5005, Australia.
New Phytol. 2004 Jul;163(1):187-200. doi: 10.1111/j.1469-8137.2004.01095.x.
• A survey of 12 plants colonized by six species of arbuscular mycorrhizal fungi was conducted to explore the diversity of Arum and Paris mycorrhizal structures. • Surveyed root material was sectioned both longitudinally and transversely, double-stained and mycorrhizal structures were identified. A detailed time course experiment using four plant, and four fungal species, was used to investigate the sequential development of hyphae, arbuscules, hyphal coils, arbusculate coils and vesicles. • The survey indicated that there was a continuum of mycorrhizal structures ranging from Arum to Paris, depending upon both the host plant and the fungus. The time course showed that total colonization increased, and that the establishment of the various mycorrhizal structures did not appear to change greatly over time. • It was concluded that identification of fungal structures and their subsequent development into morphological types is not easily defined. Visual inspection of root squashes is not always adequate, especially where transverse sections are needed to determine if longitudinal hyphae are inter or intracellular; this is essential to distinguish intermediate types.
• 对12种被6种丛枝菌根真菌定殖的植物进行了调查，以探究天南星型和泡囊型菌根结构的多样性。
• 将调查的根材料进行纵向和横向切片，双重染色并鉴定菌根结构。使用四种植物和四种真菌物种进行了详细的时间进程实验，以研究菌丝、丛枝、菌丝线圈、丛枝线圈和泡囊的连续发育过程。
• 调查表明，根据寄主植物和真菌的不同，存在一个从天南星型到泡囊型的连续菌根结构。时间进程显示，总定殖率增加，并且各种菌根结构的建立似乎不会随时间发生很大变化。
• 得出的结论是，真菌结构及其随后发育成形态类型的鉴定并不容易界定。对根压片的目视检查并不总是足够的，特别是在需要横切片来确定纵向菌丝是细胞间还是细胞内的情况下；这对于区分中间类型至关重要。
