Cavagnaro T R, Smith F A, Lorimer M F, Haskard K A, Ayling S M, Smith S E
Department of Soil and Water, University of Adelaide, Waite Campus, PMB 1, Glen Osmond, South Australia 5064, Australia.
The Centre for Plant Root Symbioses, University of Adelaide, Waite Campus, PMB 1, Glen Osmond, South Australia 5064, Australia.
New Phytol. 2001 Jan;149(1):105-113. doi: 10.1046/j.1469-8137.2001.00001.x.
• Arum- and Paris-type symbioses are the two main morphological types of arbuscular mycorrhiza. Here, the developmental time-course of the Paris-type association formed from colonization of Asphodelus fistulosus (onion weed) by Glomus coronatum is presented. • Development was monitored over 27 d. Root colonization was assessed using a modification of the magnified intersects technique (MIT), for investigating the interdependence (thus IMIT) of structures. • Hyphal and arbusculate coils were found predominantly in the outer and inner cortex of the root, respectively. The interdependence of external hyphae, hyphal coils and arbusculate coils was determined during the relatively slow development of the symbiosis. • The time required for development of Paris-type arbuscular mycorrhizas is slower than for the Arum type, and both time and space influence the formation of hyphal coils. Use of IMIT for scoring colonization allows determination of the interdependence of different fungal structures, and thus the technique has potentially wide applications, such as in relating the presence of different structures to signals from molecular probes.
• 天南星型和类南星型共生是丛枝菌根的两种主要形态类型。本文展示了由冠球囊霉（Glomus coronatum）定殖于瘘管阿福花（Asphodelus fistulosus，葱芥）形成的类南星型共生关系的发育时间进程。
• 在27天内对发育过程进行监测。采用改良的放大交叉技术（MIT）评估根系定殖情况，以研究结构之间的相互依存关系（即IMIT）。
• 菌丝圈和丛枝状线圈分别主要出现在根的外皮层和内皮层。在共生关系相对缓慢的发育过程中确定了外部菌丝、菌丝圈和丛枝状线圈之间的相互依存关系。
• 类南星型丛枝菌根的发育所需时间比天南星型慢，时间和空间都会影响菌丝圈的形成。使用IMIT对定殖情况进行评分可以确定不同真菌结构之间的相互依存关系，因此该技术具有潜在的广泛应用，例如将不同结构的存在与分子探针发出的信号联系起来。
