Department of Botany, University of Guelph, Guelph, Ontario, N1G 2W1 Canada.
Mycologia. 2002 Jul-Aug;94(4):587-95. doi: 10.1080/15572536.2003.11833187.
Two major types of arbucular mycorrhizal associations, the Arum-type and the Paris-type, have been identified based on morphological features. Although the Paris-type is the most common, it is the Arum-type that has been most intensively studied in terms of structure/function because of its prevalence in agronomically important plant species. In this study, the interface between the host cell cytoplasm and intracellular hyphae (extensive hyphal coils and arbusculate coils), which typify the Paris-type mycorrhiza, was studied. Using immunofluorescence techniques combined with laser scanning confocal microscopy, dramatic changes in the cytoskeleton in colonized cells were observed. Changes in the positioning of both host cell microtubules and actin filaments occurred in colonized plant cells. Both microtubules and actin filaments were associated with the hyphal coils and the arbusculate coils. An interfacial matrix, of host origin, was demonstrated between hyphal coils and arbusculate coils using various affinity techniques. It formed an apoplastic compartment consisting of cellulose and pectins between the fungus and host cell cytoplasm. There was less labelling adjacent to the fine branches of arbusculate coils compared to the hyphal coils. These observations show some similarities to those seen with Arum-type mycorrhizas.
两种主要的丛枝菌根共生类型，即 Arum 型和 Paris 型，已经基于形态特征被鉴定出来。尽管 Paris 型是最常见的，但由于其在农业上重要的植物物种中的普遍性，Arum 型在结构/功能方面得到了最深入的研究。在这项研究中，研究了宿主细胞质和细胞内菌丝（广泛的菌丝线圈和丛枝线圈）之间的界面，这是典型的 Paris 型菌根的特征。使用免疫荧光技术结合激光共聚焦显微镜观察到，在被感染的细胞中，细胞骨架发生了剧烈的变化。被感染的植物细胞中宿主细胞微管和肌动蛋白丝的定位都发生了变化。微管和肌动蛋白丝都与菌丝线圈和丛枝线圈相关联。使用各种亲和技术证明，在菌丝线圈和丛枝线圈之间存在一个由宿主来源的界面基质。它在真菌和宿主细胞质之间形成了一个由纤维素和果胶组成的质外体隔室。与菌丝线圈相比，在丛枝线圈的细支附近的标记较少。这些观察结果与 Arum 型菌根的观察结果有一些相似之处。
