Zhang Yalin, Zhao Lihong, Feng Zili, Guo Hongfu, Feng Hongjie, Yuan Yuan, Wei Feng, Zhu Heqin
State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China.
Puyang City Academy of Agricultural Sciences, Puyang 457000, China.
Pathogens. 2021 Jan 19;10(1):81. doi: 10.3390/pathogens10010081.
Kleb., the causal pathogen of vascular wilt, can seriously reduce the yield and quality of many crops, including cotton (). To control the harm caused by , considering the environmental pollution of chemical fungicides and their residues, the strategy of plant nutrition regulation is becoming increasingly important as an eco-friendly method for disease control. A new compound micronutrient fertilizer (CMF) found in our previous study could reduce the damage of cotton Verticillium wilt and increase yield. However, there is little information about the mode of action of CMF to control this disease. In the present study, we evaluated the role of CMF against and its mechanism of action in vitro and in vivo. In the laboratory tests, we observed that CMF could inhibit hyphal growth, microsclerotia germination, and reduce sporulation of . Further studies revealed that the biomass of in the root and hypocotyl of cotton seedlings treated with CMF were significantly reduced compared with the control, and these results could explain the decline in the disease index of cotton Verticillium wilt. Furthermore, those key genes involved in the phenylpropanoid metabolism pathway, resistance-related genes defense, and nitric oxide signaling pathway were induced in cotton root and hypocotyl tissue when treated with CMF. These results suggest that CMF is a multifaceted micronutrient fertilizer with roles in inhibiting the growth, development, and pathogenicity of and promoting cotton growth.
黄萎病菌（Kleb.）是导致维管束萎蔫病的病原体，会严重降低包括棉花在内的许多作物的产量和品质（）。为了控制其造成的危害，考虑到化学杀菌剂对环境的污染及其残留问题，植物营养调控策略作为一种环保的病害防治方法正变得越来越重要。我们之前的研究中发现的一种新型复合微肥（CMF）可以减轻棉花黄萎病的危害并提高产量。然而，关于CMF防治这种病害的作用方式的信息却很少。在本研究中，我们评估了CMF对黄萎病菌（Kleb.）的作用及其在体外和体内的作用机制。在实验室测试中，我们观察到CMF可以抑制菌丝生长、微菌核萌发并减少黄萎病菌（Kleb.）的产孢量。进一步的研究表明，与对照相比，用CMF处理的棉花幼苗根和下胚轴中的黄萎病菌（Kleb.）生物量显著降低，这些结果可以解释棉花黄萎病病情指数的下降。此外，当用CMF处理时，棉花根和下胚轴组织中参与苯丙烷代谢途径、抗性相关基因防御和一氧化氮信号通路的那些关键基因被诱导。这些结果表明，CMF是一种多方面的微肥，具有抑制黄萎病菌（Kleb.）生长、发育和致病性以及促进棉花生长的作用。
