J Biomed Nanotechnol. 2021 Mar 1;17(3):399-406. doi: 10.1166/jbn.2021.3057.
Pelvic organ prolapse (POP) has become one of the most common serious diseases affecting parous women. Weakening of pelvic ligaments plays an essential role in the pathophysiology of POP. Currently, synthetic materials are widely applied for pelvic reconstructive surgery. However, synthetic nondegradable meshes for POP therapy cannot meet the clinical requirements due to its poor biocompatibility. Herein, we fabricated electrospun core-shell nanofibers of poly(l-lactic acid)-hyaluronic acid (PLLA/HA). After that, we combined them with mouse bone marrow-derived mesenchymal stem cells (mBMSCs) to assess the cellular response and pelvic ligament tissue engineering . The cellular responses on the composite nanofibers showed that the core-shell structure nanofibers displayed with excellent biocompatibility and enhanced cellular activity without cytotoxicity. Moreover, compared with PLLA nanofibers seeded with mBMSCs, PLLA/HA nanofibers exhibited more cellular function, as revealed by the quantitative real-time polymerase chain reaction (RT-qPCR) for pelvic ligament-related gene markers including Col1a1, Col1a3 and Tnc. These features suggested that this novel core-shell nanofiber is promising in stem cell-based tissue engineering for pelvic reconstruction.
盆腔器官脱垂（POP）已成为影响经产妇的最常见严重疾病之一。盆腔韧带的弱化在 POP 的病理生理学中起着重要作用。目前，合成材料广泛应用于盆腔重建手术。然而，用于 POP 治疗的合成不可降解网片由于其生物相容性差，不能满足临床要求。在此，我们制备了聚（L-乳酸）-透明质酸（PLLA/HA）的电纺核壳纳米纤维。然后，我们将它们与小鼠骨髓间充质干细胞（mBMSCs）结合，以评估细胞反应和盆腔韧带组织工程。在复合纳米纤维上的细胞反应表明，核壳结构纳米纤维具有优异的生物相容性和增强的细胞活性，没有细胞毒性。此外，与接种 mBMSCs 的 PLLA 纳米纤维相比，PLLA/HA 纳米纤维表现出更多的细胞功能，如定量实时聚合酶链反应（RT-qPCR）检测到与骨盆韧带相关的基因标志物 Col1a1、Col1a3 和 Tnc 的表达。这些特征表明，这种新型核壳纳米纤维在基于干细胞的盆腔重建组织工程中具有广阔的应用前景。
