Shamsi M, Karimi M, Ghollasi M, Nezafati N, Shahrousvand M, Kamali M, Salimi A
Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Department of Nanotechnology and Advanced Materials, Materials and Energy Research Center, Karaj, Iran.
Mater Sci Eng C Mater Biol Appl. 2017 Sep 1;78:114-123. doi: 10.1016/j.msec.2017.02.165. Epub 2017 Mar 1.
Electrospinning method was employed for fabrication of SiO-CaO-PO bioactive glass (BG) nanofibers, poly-l-lactic acid (PLLA) nanofibers and nanocomposite scaffolds fabricated from as-prepared nanofibers. Characterization of the prepared nanofibers and scaffolds by XRD, FTIR, and SEM techniques revealed the formation of nanofibers with mean diameter of about 500nm and fully fibrous scaffolds with porous structure and interconnected pores. The growth, viability and proliferation of cultured human bone marrow mesenchymal stem cells in the fabricated nanofibers and bioactive glass-poly-l-lactic acid (BG-PLLA) nanocomposite scaffolds were studied using various biological assays including MTT, ALP activity, calcium deposit content, Alizarin red staining, and RT-PCR test. Based on the obtained results, incorporation of BG nanofibers in the nanocomposite scaffolds causes the better biological behavior of the scaffolds. In addition, three-dimensional and fibrous-porous structure of the scaffolds further contributes to their improved cell behavior compared to the components.
采用静电纺丝法制备了SiO-CaO-PO生物活性玻璃（BG）纳米纤维、聚左旋乳酸（PLLA）纳米纤维以及由上述制备的纳米纤维制成的纳米复合支架。通过X射线衍射（XRD）、傅里叶变换红外光谱（FTIR）和扫描电子显微镜（SEM）技术对制备的纳米纤维和支架进行表征，结果显示形成了平均直径约为500nm的纳米纤维以及具有多孔结构和相互连通孔隙的全纤维支架。使用包括MTT、碱性磷酸酶（ALP）活性、钙沉积含量、茜素红染色和逆转录-聚合酶链反应（RT-PCR）测试在内的各种生物学检测方法，研究了培养的人骨髓间充质干细胞在制备的纳米纤维和生物活性玻璃-聚左旋乳酸（BG-PLLA）纳米复合支架中的生长、活力和增殖情况。基于所得结果，在纳米复合支架中掺入BG纳米纤维可使支架具有更好的生物学性能。此外，与各组分相比，支架的三维纤维多孔结构进一步有助于改善其细胞行为。
