Department of Biology, Stanford University, Stanford, CA 94305-5020;
Department of Biology, Stanford University, Stanford, CA 94305-5020.
Proc Natl Acad Sci U S A. 2021 Apr 27;118(17). doi: 10.1073/pnas.2021682118.
In many developmental contexts, cell lineages have variable or flexible potency to self-renew. What drives a cell to exit from a proliferative state and begin differentiation, or to retain the capacity to divide days or years later is not clear. Here we exploit the mixed potential of the stomatal lineage ground cell (SLGC) in the leaf epidermis as a model to explore how cells might balance potential to differentiate with a reentry into proliferation. By generating transcriptomes of fluorescence-activated cell sorting-isolated populations that combinatorically define SLGCs and integrating these data with other stomatal lineage datasets, we find that SLGCs appear poised between proliferation and endoreduplication. Furthermore, we found the RNA polymerase II-related mediator complex interactor DEK and the transcription factor MYB16 accumulate differentially in the stomatal lineage and influence the extent of cell proliferation during leaf development. These findings suggest that SLGC latent potential is maintained by poising of the cell cycle machinery, as well as general and site-specific gene-expression regulators.
在许多发育背景下，细胞谱系具有可变或灵活的自我更新能力。是什么驱动细胞退出增殖状态并开始分化，或者保留数天或数年后分裂的能力尚不清楚。在这里，我们利用叶片表皮中气孔谱系基础细胞 (SLGC) 的混合潜能作为模型来探索细胞如何平衡分化潜能与重新进入增殖的能力。通过对荧光激活细胞分选分离的群体进行转录组生成，这些群体组合定义了 SLGC，并将这些数据与其他气孔谱系数据集整合，我们发现 SLGC 似乎在增殖和内复制之间处于平衡状态。此外，我们发现 RNA 聚合酶 II 相关中介复合物相互作用因子 DEK 和转录因子 MYB16 在气孔谱系中差异积累，并影响叶片发育过程中细胞增殖的程度。这些发现表明，SLGC 的潜在潜力是通过细胞周期机制的平衡以及一般和特定于位点的基因表达调节剂来维持的。
