Vanamamalai Venkata Krishna, Garg Priyanka, Kolluri Gautham, Gandham Ravi Kumar, Jali Itishree, Sharma Shailesh
National Institute of Animal Biotechnology (NIAB), Opposite Journalist Colony, Near Gowlidoddi Extended Q City Road, Gachibowli, Hyderabad, Telangana, 500032, India.
ICAR-Central Avian Research Institute, Izatnagar, Bareilly, Uttar Pradesh, 243122, India.
Sci Rep. 2021 Apr 19;11(1):8486. doi: 10.1038/s41598-021-88029-6.
Long non-coding RNAs (lncRNAs) are the transcripts of length longer than 200 nucleotides. They are involved in the regulation of various biological activities. Leghorn and Fayoumi breeds of Gallus gallus were known to be having differential resistance against Newcastle Disease Virus (NDV) infection. Differentially expressed genes which were thought to be involved in this pattern of resistance were already studied. Here we report the analysis of the transcriptomic data of Harderian gland of Gallus gallus for studying the lncRNAs involved in regulation of these genes. Using bioinformatics approaches, a total of 37,411 lncRNAs were extracted and 359 lncRNAs were differentially expressing. Functional annotation using co-expression analysis revealed the involvement of lncRNAs in the regulation of various pathways. We also identified 1232 quantitative trait loci (QTLs) associated with the genes interacting with lncRNA. Additionally, we identified the role of lncRNAs as putative micro RNA precursors, and the interaction of differentially expressed Genes with transcription factors and micro RNAs. Our study revealed the role of lncRNAs during host response against NDV infection which would facilitate future experiments in unravelling regulatory mechanisms of development in the genetic improvement of the susceptible breeds of Gallus gallus.
长链非编码RNA（lncRNAs）是长度超过200个核苷酸的转录本。它们参与多种生物活性的调控。已知来航鸡和法尤米鸡品种对新城疫病毒（NDV）感染具有不同的抗性。已经对被认为参与这种抗性模式的差异表达基因进行了研究。在此，我们报告对鸡哈德氏腺转录组数据的分析，以研究参与这些基因调控的lncRNAs。使用生物信息学方法，共提取了37411个lncRNAs，其中359个lncRNAs存在差异表达。通过共表达分析进行功能注释揭示了lncRNAs参与多种途径的调控。我们还鉴定了与与lncRNA相互作用的基因相关的1232个数量性状位点（QTLs）。此外，我们确定了lncRNAs作为假定的微小RNA前体的作用，以及差异表达基因与转录因子和微小RNA的相互作用。我们的研究揭示了lncRNAs在宿主对NDV感染的反应中的作用，这将有助于未来开展实验，以阐明鸡易感品种遗传改良中发育调控机制。
