Chen Ying, Pei Peng, Lei Zuhai, Zhang Xin, Yin Dongrui, Zhang Fan
Department of Chemistry, State Key Laboratory of Molecular Engineering of Polymers and iChem, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai, 200433, China.
Angew Chem Int Ed Engl. 2021 Jul 12;60(29):15809-15815. doi: 10.1002/anie.202103071. Epub 2021 Jun 9.
Kidney disease is usually "silent" at the early stage but can lead to severe kidney failure later on. The development of bioimaging probes with rapid distribution and long-term retention in the kidney is significant for the precise diagnosis of renal diseases. Here, a strategy for the peptide-mediated delivery and long-term accumulation (>48 h) of second near-infrared window (NIR-II) fluorophores into the kidney is demonstrated. It is shown that both the hepatic-cleared organic molecules and fast renal-cleared ultrasmall nanoparticles can be retained in the kidney after conjugation to the peptide with high polarity. Moreover, a ROS-responsive activatable bilateral NIR-II sensor was designed based on the kidney targeting peptide, which enables both in vivo long-term kidney monitoring and in vitro urine analysis. The capability of the peptide-based sensor to detect early kidney injury and report on kidney dysfunctional progression is particularly crucial for chemotherapy regimen optimization and timely renoprotective intervention during medication.
肾脏疾病在早期通常是“无症状的”，但后期可能会导致严重的肾衰竭。开发在肾脏中快速分布并长期滞留的生物成像探针对于肾脏疾病的精确诊断具有重要意义。在此，展示了一种肽介导的将第二近红外窗口（NIR-II）荧光团递送至肾脏并长期积累（>48小时）的策略。结果表明，经肝脏清除的有机分子和经肾脏快速清除的超小纳米颗粒在与高极性肽缀合后均可保留在肾脏中。此外，基于肾脏靶向肽设计了一种ROS响应型可激活双侧NIR-II传感器，它能够实现体内长期肾脏监测和体外尿液分析。基于肽的传感器检测早期肾脏损伤并报告肾脏功能障碍进展的能力对于化疗方案优化以及用药期间及时的肾脏保护干预尤为关键。
