Department of Chemistry and Molecular Biology, University of Gothenburg, Kemivägen 10, 41296, Gothenburg, Sweden.
Faculty of Health and Medical Sciences, School of Biosciences and Medicine, University of Surrey, Guildford, Surrey, GU2 7XH, UK.
Angew Chem Int Ed Engl. 2021 Jul 5;60(28):15302-15306. doi: 10.1002/anie.202104308. Epub 2021 Jun 9.
Stress granules (SGs) are membrane-less organelles that assemble in the cytoplasm to organize cellular contents and promote rapid adaptation during stress. To understand how SGs contribute to physiological functions, we used electrochemical measurements to detect electroactive species in SGs. With amperometry, we discovered that reactive oxygen species (ROS) are encapsulated inside arsenite-induced SGs, and H O is the main species. The release kinetics of H O from single SGs and the number of H O molecules were quantified. The discovery that SGs contain ROS implicates them as communicators of the cellular stresses rather than a simple endpoint. This may explain how SGs regulate cellular metabolism and stress responses. This may also help better understand their cytoprotective functions in pathological conditions associated with SGs such as neurodegenerative diseases (NDs), cancers and viral infections.
应激颗粒（SGs）是细胞质中无膜的细胞器，它们组装在一起以组织细胞内容物，并在应激期间促进快速适应。为了了解 SGs 如何促进生理功能，我们使用电化学测量来检测 SGs 中的电化学活性物质。通过安培法，我们发现亚砷酸盐诱导的 SGs 内包裹着活性氧（ROS），而 H O 是主要物质。我们还定量了 H O 从单个 SGs 中的释放动力学和 H O 分子的数量。发现 SGs 中含有 ROS，这意味着它们是细胞应激的通讯者，而不仅仅是一个简单的终点。这可能解释了 SGs 如何调节细胞代谢和应激反应。这也可能有助于更好地理解它们在与 SGs 相关的病理条件下的细胞保护功能，如神经退行性疾病（NDs）、癌症和病毒感染。
