School of Life Science and Technology, Wuhan Polytechnic University, Wuhan, 430023, China.
National R&D Center for Se-Rich Agricultural Products Processing, School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan, 430023, China.
Glycoconj J. 2022 Jun;39(3):369-380. doi: 10.1007/s10719-022-10058-8. Epub 2022 Apr 13.
Morchella is the famous medicinal fungi in the ascomycetes. In this study, a new water-soluble polysaccharide (MSP-3-1) with an average molecular weight of 2.35 × 10 Da was extracted and purified from fruiting bodies of cultivated M. Sextelata. The structural characterization and biological activities of purified polysaccharide was further investigated. The results indicated that MSP-3-1 was mainly a α-glucan, mainly consisting of mannose (Man), glucose (Glc) and galactose (Gal) in a ratio of 5.10: 91.39: 3.51. Its surface morphology exhibited irregular lamellar structures with small voids. And the particle size analysis showed that MSP-3-1 was the homogeneous nanoparticle in water solution. Furthermore, the antioxidant activity analysis showed that MSP-3-1 possessed certain scavenging activity against hydroxyl radicals, DPPH radicals and ABTS radicals in a dose-dependent manner. Immunological tests suggested that MSP-3-1 could significantly promote the proliferation, phagocytosis and nitric oxide (NO) production of macrophage RAW264.7. Thus, our results will provide a theoretical basis for the development and utilization of Morchella Sextelata polysaccharides as an immunmodulatory component in functional foods.
羊肚菌是子囊菌中著名的药用真菌。本研究从栽培羊肚菌子实体中提取并纯化得到一种新的水溶性多糖（MSP-3-1），其平均分子量为 2.35×10 Da。进一步研究了纯化多糖的结构特征和生物活性。结果表明，MSP-3-1 主要是一种α-葡聚糖，主要由甘露糖（Man）、葡萄糖（Glc）和半乳糖（Gal）组成，摩尔比为 5.10:91.39:3.51。其表面形态呈现不规则的层状结构，有小的空隙。颗粒大小分析表明，MSP-3-1 在水溶液中是均一的纳米颗粒。此外，抗氧化活性分析表明，MSP-3-1 对羟自由基、DPPH 自由基和 ABTS 自由基具有一定的清除活性，呈剂量依赖性。免疫试验表明，MSP-3-1 能显著促进巨噬细胞 RAW264.7 的增殖、吞噬作用和一氧化氮（NO）的产生。因此，我们的研究结果为羊肚菌多糖作为功能性食品中的免疫调节成分的开发和利用提供了理论依据。
