环状 RNA circ_0008285 通过调节滋养细胞的生物学功能在妊娠期糖尿病中的作用。
The role of circular RNA circ_0008285 in gestational diabetes mellitus by regulating the biological functions of trophoblasts.
机构信息
Department of Obstetrics and Gynecology, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, 510080, China.
出版信息
Biol Res. 2021 Apr 20;54(1):14. doi: 10.1186/s40659-021-00337-3.
BACKGROUND
Circular RNAs (circRNAs) has emerged as vital regulator involved in various diseases. In this study, we identified and investigated the potential circRNAs involved in gestational diabetes mellitus (GDM).
METHODS
High-throughput sequencing was used to collect the plasma circRNAs expression profiles of GDM patients. Quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) was used to measure the expressions of circ_0008285 and circ_0001173 in the plasma specimens. The Pearson's correlation test was employed to assess the correlation between 2 circRNAs expression and the clinicopathologic data. Two circRNAs expression was verified in high glucose (HG)-induced HTR-8/SVneo cells. MTS, transwell assay was used to evaluate the effects of circ_0008285 expression on HG-induced HTR-8/SVneo cells. The network of circ_0008285 was constructed using cytocape.
RESULTS
In GDM patients, the expression of circ_0008285 was significantly upregulated, while that of circ_0001173 was decreased. Circ_0008285 was significantly correlated with the total cholesterol and LDL-C levels. Circ_0001173 was significantly correlated with glycated hemoglobin. HG promoted the proliferation, invasion, and migration in HTR-8/SVneo cells, while the knockdown of circ_0008285 exerted reverse effects. In addition, network construction exhibited that circ_0008285 had 45 miRNA binding sites, which correlated with 444 mRNA.
CONCLUSIONS
circ_0008285 plays an important role and provides a clue for the usage of therapeutic targets in the development of GDM.
背景
环状 RNA（circRNAs）已成为参与多种疾病的重要调控因子。在这项研究中，我们鉴定并研究了与妊娠期糖尿病（GDM）相关的潜在环状 RNA。
方法
采用高通量测序技术收集 GDM 患者血浆环状 RNA 表达谱。采用实时定量逆转录聚合酶链反应（qRT-PCR）测量血浆标本中 circ_0008285 和 circ_0001173 的表达。采用 Pearson 相关检验评估 2 个 circRNA 表达与临床病理数据之间的相关性。在高糖（HG）诱导的 HTR-8/SVneo 细胞中验证 2 个 circRNAs 的表达。采用 MTS 和 Transwell 实验评估 circ_0008285 表达对 HG 诱导的 HTR-8/SVneo 细胞的影响。采用 Cytocape 构建 circ_0008285 网络。
结果
在 GDM 患者中，circ_0008285 的表达显著上调，而 circ_0001173 的表达则降低。circ_0008285 与总胆固醇和 LDL-C 水平显著相关。circ_0001173 与糖化血红蛋白显著相关。HG 促进 HTR-8/SVneo 细胞的增殖、侵袭和迁移，而 circ_0008285 的敲低则产生相反的效果。此外，网络构建表明 circ_0008285 有 45 个 miRNA 结合位点，与 444 个 mRNA 相关。
结论
circ_0008285 发挥着重要作用，并为 GDM 发展中治疗靶点的应用提供了线索。
Zotero 插件