Sugitani Yoshihiko, Inoue Ryo, Inatomi Osamu, Nishida Atsushi, Morishima So, Imai Takayuki, Kawahara Masahiro, Naito Yuji, Andoh Akira
Department of Medicine, Shiga University of Medical Science, Seta-Tsukinowa, Otsu, Shiga 520-2192, Japan.
Laboratory of Animal Science, Department of Applied Biological Sciences, Faculty of Agriculture, Setsunan University, Nagaotoge-cho 45-1, Hirakata, Osaka 573-0101, Japan.
J Clin Biochem Nutr. 2021 Mar;68(2):187-192. doi: 10.3164/jcbn.20-93. Epub 2020 Sep 30.
The number of patients with chronic constipation is increasing in Japan. We investigated the gut mucosa-associated microbiome in Japanese patients with functional constipation. Diagnosis was made according to the Rome IV criteria. Mucosal samples were obtained by gentle brushing of mucosa surfaces. The gut microbiome was analyzed using 16S rRNA gene sequencing. There were no significant differences in bacteria α-diversity such as richness and evenness. The PCoA indicated significant structural differences between the constipation group and healthy controls ( = 0.017 for unweighted and = 0.027 for weighted). The abundance of the phylum Bacteroidetes was significantly higher in the constipation group. The abundance of the genera , , , and was significantly higher in the constipation group. The abundance of the genera , , , , and was significantly lower in the constipation group. In the constipation group, the proportion of genes responsible for sulfur metabolism, selenocompound metabolism, sulfur relay system was significantly higher and the proportion of d-arginine and d-ornithine metabolism and flavonoid biosynthesis was significantly lower. In conclusion, we identified differences of the mucosa-associated microbiome between Japanese patients with functional constipation and healthy controls. The mucosa-associated microbiome of functional constipation was characterized by higher levels of Bacteroidetes ().
在日本，慢性便秘患者的数量正在增加。我们对日本功能性便秘患者的肠道黏膜相关微生物群进行了调查。诊断依据罗马IV标准进行。通过轻柔擦拭黏膜表面获取黏膜样本。使用16S rRNA基因测序分析肠道微生物群。在细菌α多样性（如丰富度和均匀度）方面没有显著差异。主坐标分析（PCoA）表明便秘组与健康对照组之间存在显著的结构差异（非加权分析P = 0.017，加权分析P = 0.027）。便秘组中拟杆菌门的丰度显著更高。便秘组中属、、、和的丰度显著更高。便秘组中属、、、、和的丰度显著更低。在便秘组中，负责硫代谢、硒化合物代谢、硫传递系统的基因比例显著更高，而d-精氨酸和d-鸟氨酸代谢以及类黄酮生物合成的比例显著更低。总之，我们确定了日本功能性便秘患者与健康对照组之间黏膜相关微生物群的差异。功能性便秘的黏膜相关微生物群的特征是拟杆菌门（）水平较高。
