中国山东大蒜和蚜虫中草木樨状黄芪矮化病毒及其相关卫星α病毒的全基因组分析。
Genome-wide analysis of milk vetch dwarf virus and related alphasatellites from garlic and aphids in Shandong Province, China.
机构信息
College of Plant Protection, Shandong Agricultural University, Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, Collaborative Innovation Center of Fruit and Vegetable Quality and Efficient Production in Shandong, Taian, 271018, China.
Plant Protection Station of Shandong Province, Jinan, 250100, China.
出版信息
Arch Virol. 2022 Oct;167(10):2071-2077. doi: 10.1007/s00705-022-05490-y. Epub 2022 Jul 7.
In 2019 and 2020, symptoms of dwarfing, yellowing, and reddening were observed in garlic in open fields in Shandong Province, China. Milk vetch dwarf virus (MDV) was detected in aphids and symptomatic garlic plants using polymerase chain reaction analysis. Furthermore, it was demonstrated using an aphid transmission test that garlic is a natural host of MDV. Rolling-circle amplification was combined with the use of specific primers to amplify the complete genomes of MDV and its related alphasatellites. This is the first report of complete genome sequences of MDV and related alphasatellites from garlic and aphid samples.
2019 年和 2020 年，中国山东省露天大蒜出现矮化、黄化和变红症状。利用聚合酶链反应分析在蚜虫和有症状的大蒜植株中检测到了大蒜矮化病毒（MDV）。此外，蚜虫传播试验证明大蒜是 MDV 的天然宿主。采用滚环扩增技术结合使用特异性引物对 MDV 及其相关的卫星α 病毒的完整基因组进行了扩增。这是首次从大蒜和蚜虫样本中报告 MDV 及其相关卫星α 病毒的完整基因组序列。
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