贝宁一名 HIV-1 感染者的诊断中经典聚合酶链反应的贡献：病例报告。
Contribution of the classical polymerase chain reaction in the diagnosis of a HIV-1 infected patient in Benin: a case report.
机构信息
Laboratory of Cell Biology and Physiology, Department of Biochemistry and Cellular Biology, Faculty of Sciences and Technology (FAST), Institute of Applied Biomedical Sciences (ISBA), University of Abomey-Calavi, Calavi, 01 BP 918, Cotonou, Benin.
National Reference Laboratory of Health Program Fighting Against AIDS in Benin (LNR/PSLS), Health Ministry of Benin, BP 1258, Cotonou, Benin.
出版信息
AIDS Res Ther. 2021 Apr 21;18(1):18. doi: 10.1186/s12981-021-00342-2.
BACKGROUND
First ambitious target by 2020 of UNAIDS is that 90% of people living with HIV know their HIV status. In people older than 18 months of age, serological confirmation test is recommended to confirm HIV infection.
CASE PRESENTATION
Here we report the case of a patient tested positive with HIV-1, ELISA, Murex Ag⁄Ab Combination assay (OD450 = 0.802 and cutoff-OD = 0.279) and negative by using FIRST RESPONSE HIV1-2.O CARD TEST (version 2.0) RAPID HIV CARD TEST. Viral load performed with Cobas TaqMan 96/Cobas Ampliprep was 6.49log The virus could be sequenced in partial gag and pol genes and belonged to CRF02_AG clade.
CONCLUSION
Conventional PCR is a complementary method for the diagnosis of inconclusive HIV-1 serologies by antibodies.
背景
联合国艾滋病规划署（UNAIDS）的 2020 年首要目标是使 90%的艾滋病毒感染者了解其艾滋病毒状况。对于 18 个月以上的人群，建议使用血清学确认试验来确认艾滋病毒感染。
病例介绍
在此，我们报告了一例患者的 HIV-1 酶联免疫吸附试验（ELISA）、Murex Ag⁄Ab 组合检测（OD450=0.802，临界 OD=0.279）阳性，而使用 FIRST RESPONSE HIV1-2.O CARD TEST（版本 2.0）RAPID HIV CARD TEST 检测为阴性。采用 Cobas TaqMan 96/Cobas Ampliprep 进行的病毒载量检测为 6.49log。可对部分 gag 和 pol 基因进行测序，该病毒属于 CRF02_AG 分支。
结论
对于抗体不确定的 HIV-1 血清学检测，常规 PCR 是一种补充诊断方法。
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