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评价 Gen-Probe Aptima HIV-1 RNA 定性检测作为替代 Western blot 分析用于确认 HIV 感染的方法。

Evaluation of the Gen-Probe Aptima HIV-1 RNA qualitative assay as an alternative to Western blot analysis for confirmation of HIV infection.

机构信息

Division of Infectious Diseases, Department of Pediatrics, Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA.

出版信息

J Clin Microbiol. 2011 Apr;49(4):1642-5. doi: 10.1128/JCM.02183-10. Epub 2011 Feb 23.

DOI:10.1128/JCM.02183-10
PMID:21346052
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3122829/
Abstract

The Gen-Probe Aptima HIV-1 RNA qualitative assay was evaluated as an alternative to Western blot analysis for the confirmation of HIV infection using serum samples that were repeatedly reactive for HIV antibodies. The Aptima HIV assay readily discriminated between HIV-1-infected and -uninfected individuals and effectively reduced the number of indeterminate results relative to Western blot analysis.

摘要

基因探针 Aptima HIV-1 RNA 定性检测被评估为 Western blot 分析的替代方法，用于确认血清样本中 HIV 抗体反复呈阳性的 HIV 感染。Aptima HIV 检测能够很好地区分 HIV-1 感染和未感染个体，并与 Western blot 分析相比，有效减少了不确定结果的数量。

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Ultra-high-throughput, automated nucleic acid detection of human immunodeficiency virus (HIV) for infant infection diagnosis using the Gen-Probe Aptima HIV-1 screening assay.使用Gen-Probe Aptima HIV-1筛查检测法对人类免疫缺陷病毒（HIV）进行超高通量自动化核酸检测，用于婴儿感染诊断。
J Clin Microbiol. 2009 Aug;47(8):2465-9. doi: 10.1128/JCM.00317-09. Epub 2009 May 27.
3
Revised surveillance case definitions for HIV infection among adults, adolescents, and children aged <18 months and for HIV infection and AIDS among children aged 18 months to <13 years--United States, 2008.美国2008年成人、青少年及18个月以下儿童HIV感染以及18个月至不满13岁儿童HIV感染与艾滋病监测病例定义修订版
MMWR Recomm Rep. 2008 Dec 5;57(RR-10):1-12.
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Qualitative human immunodeficiency virus RNA analysis of dried blood spots for diagnosis of infections in infants.用于诊断婴儿感染的干血斑的定性人类免疫缺陷病毒RNA分析。
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