Centre for Advanced Research in Indian System of Medicine (CARISM), SASTRA Deemed University, Thirumalaisamudram, Thanjavur, 613401, India.
Bioprocess Intensification Laboratory, Centre for Bioenergy, School of Chemical and Biotechnology, SASTRA Deemed To Be University, Thirumalaisamudram, Thanjavur, 613401, India.
Sci Rep. 2021 Apr 21;11(1):8643. doi: 10.1038/s41598-021-87960-y.
The green synthesis of NPs through plant extracts can be a modest, one-pot alternative synthesis to the conventional physical or chemical method. The prime focus of this study is to produce MNPs by the reducing effect of Bauhinia tomentosa leaf extract, and it was immobilized in porcine pancreatic lipase (PPL). Synthesized NPs were characterized by field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD) and Raman spectroscopy, UV-Vis Spectrometry, Thermogravimetry, and Differential Scanning Calorimeter (DSC), Zeta potential test, VSM, BET and Fourier Transform Infrared Spectroscopy (FTIR). The effect of process parameters was studied, about the efficiency of immobilization are enzyme stability, the extent of enzyme reusability, its separation from products, the activity of immobilized enzyme, recovery, and its loss. Finally, the immobilized lipase was used for the synthesis of 1,3-diolein using enzyme-mediated esterification of oleic acid and glycerol. Under optimized condition (reaction temp-55 [Formula: see text]C; molar ratio-2.5:1; pH-7) diolein yield was achieved to be 94%. Therefore, this work was further used for the industrial production of 1,3-diacylglycerol since a perfect enzyme-catalyzed process was observed.
通过植物提取物进行 NPs 的绿色合成可以作为传统物理或化学方法的温和、一锅合成的替代方法。本研究的主要重点是通过豆科植物绒毛叶提取物的还原作用来制备 MNPs，并将其固定在猪胰腺脂肪酶（PPL）上。合成的 NPs 通过场发射扫描电子显微镜（FESEM）、X 射线衍射（XRD）和拉曼光谱、紫外-可见分光光度法、热重分析和差示扫描量热法（DSC）、Zeta 电位测试、振动样品磁强计（VSM）、BET 和傅里叶变换红外光谱（FTIR）进行了表征。研究了工艺参数的影响，关于固定化效率的是酶稳定性、酶的可重复使用程度、从产物中分离、固定化酶的活性、回收和损失。最后，将固定化脂肪酶用于通过油酸和甘油的酶介导酯化反应合成 1,3-二油精。在优化条件（反应温度为 55[Formula: see text]C；摩尔比为 2.5:1；pH 值为 7）下，二油精的产率达到 94%。因此，由于观察到了完美的酶催化过程，因此这项工作进一步用于 1,3-二酰基甘油的工业生产。
