从肯尼亚内罗毕市腹泻儿童中分离出的具有表型和基因型抗生素抗性的致泻性致病型
Phenotypic and Genotypic Antibiotic Resistant diarrheagenic pathotypes isolated from Children with Diarrhea in Nairobi City, Kenya.
作者信息
Webale Mark Kilongosi, Guyah Bernard, Wanjala Christine, Nyanga Peter Lokamar, Webale Sella K, Abonyo Collins, Kitungulu Nicholas, Kiboi Nathan, Bowen Nancy
机构信息
School of Health Sciences, Kirinyaga University, Kutus, Kenya.
School of Public Health, Maseno University, Kenya.
出版信息
Ethiop J Health Sci. 2020 Nov;30(6):881-890. doi: 10.4314/ejhs.v30i6.5.
BACKGROUND
The marked genome plasticity of diarrheagenic Escherichia coli promotes emergence of pathotypes displaying unique phenotypic and genotypic resistance. This study examined phenotypic and genotypic antibiotic resistant diarrheagenic Escherichia coli pathotypes among children in Nairobi City, Kenya.
METHODS
In a cross-sectional study, diarrheagenic Escherichia coli pathotypes were isolated from stool samples and their phenotypic and genotypic resistance against eight antimicrobial agents assayed.
RESULTS
Diarrheagenic Escherichia coli was detected in 136(36.4%) children. Most of diarrheagenic Escherichia coli that were resistant to ampicillin, ceftriaxone, streptomycin, gentamycin, ciprofloxacin, chloramphenicol, erythromycin and tetracycline, harbored citm, bla CMY, aadA1, aac(3)-IV, qnr, catA, ere(A) and tet(A) corresponding resistant genes.
CONCLUSION
Antimicrobial-resistant genes are highly prevalent among phenotypic resistant ETEC pathotypes indicating a possibility of horizontal gene transfer in spreading antibiotic resistant genes among E. coli pathotypes.
背景
致泻性大肠杆菌显著的基因组可塑性促使表现出独特表型和基因型耐药性的致病型出现。本研究调查了肯尼亚内罗毕市儿童中致泻性大肠杆菌致病型的表型和基因型抗生素耐药情况。
方法
在一项横断面研究中，从粪便样本中分离出致泻性大肠杆菌致病型，并检测其对八种抗菌药物的表型和基因型耐药性。
结果
在136名（36.4%）儿童中检测到致泻性大肠杆菌。大多数对氨苄西林、头孢曲松、链霉素、庆大霉素、环丙沙星、氯霉素、红霉素和四环素耐药的致泻性大肠杆菌携带了citm、bla CMY、aadA1、aac(3)-IV、qnr、catA、ere(A)和tet(A)相应的耐药基因。
结论
抗菌耐药基因在表型耐药的肠毒素型大肠杆菌致病型中高度流行，这表明在大肠杆菌致病型之间传播抗生素耐药基因存在水平基因转移的可能性。
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