DETECTION OF DIARRHEAGENIC IN HUMAN DIARRHEIC STOOL AND DRINKING WATER SAMPLES IN OUAGADOUGOU, BURKINA FASO.

BACKGROUND

The presence of diarrheagenic (DEC) in drinking water, is a grave public health problem. This study was aimed at characterization of diarrheagenic isolated from drinking water and faecal samples from diarrheic patients in Ouagadougou, Burkina Faso.

MATERIALS AND METHODS

A total of 242 water samples consisting of 182 potable sachets and 60 from boreholes were collected in the period between October 2018 and April 2019 in the city of Ouagadougou. Faecal samples were also collected from 201 diarrheic patients visiting National Public Health Laboratory for a biological diagnosis by coproculture. The presence of virulence genes associated with DEC was determined by 16-plex polymerase chain reaction from bacteria culture.

RESULTS

From drinking water, we found 17% (42/242) isolates in which 1% (2/242) DEC were detected. Among analyzed samples (182 sachet water versus 60 borehole water), the two DEC (01 ETEC and 01 EPEC) were detected in sachet water. DEC were detected in 20% (40/201) of patients. Enteroaggregative (EAEC) were mostly detected in 10% followed by Enteropathogenic (EPEC) in 4%, Enteroinvasive (EIEC) in 2%, and Shiga toxin-producing (STEC) 0.5%. However, Enterotoxigenic (ETEC) was not detected alone, but in co-infections with EAEC.

CONCLUSION

The present study documented the prevalence of pathovars associated in patients with diarrhea, and shows that drinking water might be a source of DEC transmission in human.