科特迪瓦阿比泻湖水体、沉积物、鱼类和螃蟹中分离出的与致泻性致病型相关的毒力基因流行情况
Prevalence of Virulence Genes Associated with Diarrheagenic Pathotypes of Isolates from Water, Sediment, Fish, and Crab in Aby Lagoon, Côte d'Ivoire.
作者信息
Kambire Ollo, Adingra Ama Antoinette, Yao Konan Mathurin, Koffi-Nevry Rose
机构信息
Department of Biochemistry and Food Sciences, University of Peleforo Gon Coulibaly, BP 1328, Korhogo, Côte d'Ivoire.
Laboratory of Microbiology, Oceanographic Research Center, BP V 18, Abidjan, Côte d'Ivoire.
出版信息
Int J Microbiol. 2017;2017:9532170. doi: 10.1155/2017/9532170. Epub 2017 Jun 6.
This study was conducted to characterize virulence genes of isolates from water, sediment, fish, and crab in Aby Lagoon. Serogrouping was performed by EPEC antisera in 113 strains. The presence of diarrhea-associated genes and was assessed by multiplex PCR using specific primers. Based on the multiplex PCR, sixty-two isolates (42 from water, 19 from sediment, and 1 from crab) were positive for virulence genes, including 34 positive for (ETEC), 46 positive for (ETEC), 24 positive for both and 6 positive for (EHEC), 1 positive for both + , and 1 positive for both + Genes (EPEC) and (EAEC) were not detected. Nine serogroups (O114, O127, O55, O111, O86, O119, O126, O128, and O142) were identified. This study revealed the presence of diarrheagenic and nondiarrheagenic and potential public health risks if fishery products are not appropriately cooked.
本研究旨在鉴定阿比泻湖水体、沉积物、鱼类和螃蟹中分离株的毒力基因。采用肠致病性大肠杆菌（EPEC）抗血清对113株菌株进行血清分型。使用特异性引物通过多重聚合酶链反应（PCR）评估腹泻相关基因的存在情况。基于多重PCR，62株分离株（42株来自水体，19株来自沉积物，1株来自螃蟹）的毒力基因呈阳性，其中34株产肠毒素大肠杆菌（ETEC）的 呈阳性，46株ETEC的 呈阳性，24株 和 均呈阳性，6株肠出血性大肠杆菌（EHEC）的 呈阳性，1株 和 均呈阳性，1株 和 均呈阳性。未检测到EPEC的基因 和肠集聚性大肠杆菌（EAEC）的基因 。鉴定出9个血清型（O114、O127、O55、O111、O86、O119、O126、O128和O142）。本研究揭示了腹泻性和非腹泻性 的存在，以及如果渔业产品烹饪不当可能存在的公共卫生风险。
Zotero 插件