Cowell R L, Fox J C, Panciera R J, Tyler R D
Department of Veterinary Pathology, College of Veterinary Medicine, Oklahoma State University, Stillwater 74078.
Vet Parasitol. 1988 Apr;28(1-2):43-52. doi: 10.1016/0304-4017(88)90017-9.
A microfluorometric immunoassay was developed for the detection of antibodies to a Cytauxzoon organism of bobcat origin. A homogenated antigen of RBC-parasite lysate was used to measure antibody levels in sera from cats experimentally infected with the non-pathogenic erythrocyte form of the bobcat Cytauxzoon organism. Antibody levels in sera collected at weekly intervals, expressed as FIAX values, were correlated with parasitemia levels and erythrocyte packed cell volume (PCV). The antibody levels tended to be highest and the PCV lowest at or near the peak parasitemias. Splenectomized cats developed higher antibody and parasitemia levels than non-splenectomized cats. Single serum samples collected from 31 cats were used to establish a baseline response level for normal domestic cat populations.
开发了一种微量荧光免疫测定法，用于检测源自山猫的梨形虫属生物体的抗体。使用红细胞-寄生虫裂解物的匀浆抗原，来测量实验感染了山猫梨形虫属生物体非致病性红细胞形式的猫血清中的抗体水平。每周采集的血清中的抗体水平，以FIAX值表示，与寄生虫血症水平和红细胞压积（PCV）相关。在寄生虫血症高峰或接近高峰时，抗体水平往往最高，而PCV最低。脾切除的猫比未脾切除的猫产生更高的抗体和寄生虫血症水平。从31只猫收集的单个血清样本用于建立正常家猫群体的基线反应水平。
