Zhong Ming-Wei, Li Yue, Cheng Yu-Gang, Liu Qiao-Ran, Hu San-Yuan, Zhang Guang-Yong
Department of General Surgery, The First Affiliated Hospital of Shandong First Medical University and Shandong Provincial Qianfoshan Hospital, Jinan 250014, Shandong Province, China.
Department of General Surgery, Shandong Qianfoshan Hospital, Cheeloo College of Medicine, Shandong University, Jinan 250014, Shandong Province, China.
World J Diabetes. 2021 Apr 15;12(4):453-465. doi: 10.4239/wjd.v12.i4.453.
Sleeve gastrectomy (SG) can induce prominent remission of type 2 diabetes mellitus. However, the long-term remission rate of diabetes usually decreases over time. Oligofructose has been verified to modulate host metabolism. The aim of this study was to explore the protective effect of oligofructose on high-fat diet (HFD)-induced metabolic dysfunction after SG.
To study the effect and mechanism of oligofructose on diabetic remission in diabetic rats after SG.
SG and SHAM operation were performed on diabetes rats induced with an HFD, nicotinamide, and low-dose streptozotocin. Then the rats in the SHAM and SG groups were continuously provided with the HFD, and the rats in sleeve gastrectomy-oligofructose group were provided with a specific HFD containing 10% oligofructose. Body weight, calorie intake, oral glucose tolerance test, homeostasis model assessment of insulin resistance, lipid profile, serum insulin, glucagon-like peptide 1 (GLP-1), total bile acids, lipopolysaccharide (LPS), and colonic microbiota levels were determined and compared at the designated time points. All statistical analyses were performed using Statistic Package for Social Science version 19.0 (IBM, United States), and the statistically significant difference was considered at < 0.05.
At 2 wk after surgery, rats that underwent SG exhibited improved indexes of glucose and lipid metabolism. Compared with the SG group, the rats from SG-oligofructose group exhibited better parameters of glucose and lipid metabolism, lower body weight (526.86 ± 21.51 469.25 ± 21.84, < 0.001), calorie intake (152.14 ± 9.48 129.63 ± 8.99, < 0.001), homeostasis model assessment of insulin resistance (4.32 ± 0.57 3.46 ± 0.52, < 0.05), and LPS levels (0.19 ± 0.01 0.16 ± 0.01, < 0.05), and higher levels of insulin (1.17 ± 0.17 1.58 ± 0.16, < 0.001) and GLP-1 (12.39 ± 1.67 14.94 ± 1.86, < 0.001), and relative abundances of (0.0034 ± 0.0014 0.0343 ± 0.0064, < 0.001), (0.0161 ± 0.0037 0.0357 ± 0.0047, < 0.001), and (0.0050 ± 0.0024 0.0507 ± 0.0100, < 0.001) at the end of the study. However, no difference in total bile acids levels was observed between the two groups.
Oligofructose partially prevents HFD-induced glucose and lipid metabolism damage after SG, which may be due to the changes of calorie intake, insulin, GLP-1, LPS, and the gut microbiota in rats.
袖状胃切除术（SG）可使2型糖尿病显著缓解。然而，糖尿病的长期缓解率通常会随时间下降。低聚果糖已被证实可调节宿主代谢。本研究旨在探讨低聚果糖对SG后高脂饮食（HFD）诱导的代谢功能障碍的保护作用。
研究低聚果糖对SG后糖尿病大鼠糖尿病缓解的作用及机制。
对用HFD、烟酰胺和低剂量链脲佐菌素诱导的糖尿病大鼠进行SG和假手术。然后，假手术组和SG组的大鼠继续给予HFD，袖状胃切除术-低聚果糖组的大鼠给予含10%低聚果糖的特定HFD。在指定时间点测定并比较体重、卡路里摄入量、口服葡萄糖耐量试验、胰岛素抵抗稳态模型评估、血脂谱、血清胰岛素、胰高血糖素样肽1（GLP-1）、总胆汁酸、脂多糖（LPS）和结肠微生物群水平。所有统计分析均使用社会科学统计软件包第19.0版（美国IBM公司）进行，差异有统计学意义为P<0.05。
术后2周，接受SG的大鼠血糖和脂质代谢指标有所改善。与SG组相比，SG-低聚果糖组的大鼠血糖和脂质代谢参数更好，体重更低（526.86±21.51对469.25±21.84，P<0.001），卡路里摄入量更低（152.14±9.48对129.63±8.99，P<0.001），胰岛素抵抗稳态模型评估更低（4.32±0.57对3.46±0.52，P<0.05），LPS水平更低（0.19±0.01对0.16±0.01，P<0.05），胰岛素水平更高（1.17±0.17对1.58±0.16，P<0.001），GLP-1水平更高（12.39±1.67对14.94±1.86，P<0.001），以及在研究结束时，（某种微生物的相对丰度，原文此处未明确微生物名称）（0.0034±0.0014对0.0343±0.0064，P<0.001）、（另一种微生物的相对丰度，原文此处未明确微生物名称）（0.0161±0.0037对0.0357±0.0047，P<0.001）和（又一种微生物的相对丰度，原文此处未明确微生物名称）（0.0050±0.0024对0.0507±0.0100，P<0.001）更高。然而，两组之间总胆汁酸水平未观察到差异。
低聚果糖可部分预防SG后HFD诱导的血糖和脂质代谢损伤，这可能是由于大鼠卡路里摄入量、胰岛素、GLP-1、LPS和肠道微生物群的变化所致。
