Department of Biology, Faculty of Science, Mahasarakham University, Maha Sarakham Province, 44150, Thailand.
Medical Biotechnology, College of Medicine and Public Health, Flinders University, Bedford Park, SA, 5042, Australia.
Arch Microbiol. 2021 Aug;203(6):3407-3413. doi: 10.1007/s00203-021-02309-3. Epub 2021 Apr 23.
A Gram-positive, aerobic, actinobacterial strain with rod-shaped spores, CAP47R, which was isolated from the surface-sterilized root of a native pine tree (Callitris preissii), grown in South Australia is described. The major cellular fatty acid of this strain was iso-H-C and major menaquinone was MK-8(H). The diagnostic diamino acid in the cell-wall peptidoglycan was identified as meso-diaminopimelic acid. These chemotaxonomic data confirmed the affiliation of strain CAP47R to the genus Pseudonocardia. Phylogenetic evaluation based on 16S rRNA gene sequence analysis placed this strain in the family Pseudonocardiaceae, being most closely related to Pseudonocardia xishanensis JCM 17906 (98.8%), Pseudonocardia oroxyli DSM 44984 (98.7%), Pseudonocardia thailandensis CMU-NKS-70 (98.7%), and Pseudonocardia ailaonensis DSM 44979 (97.9%). The results of the polyphasic study which contain genome comparisons of ANIb, ANIm, and digital DNA-DNA hybridization revealed the differentiation of strain CAP47R from the closest species with validated names. This strain represents a novel species and the name proposed for this microorganism is Pseudonocardia pini sp. nov., indicating the source of this actinobacterium from a pine tree. The type strain is CAP47R (= DSM 108967 = NRRL B-65534). Genome mining revealed that this strain contained a variety of genes encoding enzymes that can degrade hazardous chemicals.
一株革兰氏阳性、需氧、放线菌菌株 CAP47R，其具有杆状孢子，从生长在南澳大利亚的本地松树（Callitris preissii）表面消毒的根部分离得到。该菌株的主要细胞脂肪酸是 iso-H-C，主要的menaquinone 是 MK-8(H)。细胞壁肽聚糖中的诊断二氨基氨基酸被鉴定为 meso-diaminopimelic 酸。这些化学分类学数据证实了菌株 CAP47R 属于假诺卡氏菌属。基于 16S rRNA 基因序列分析的系统发育评估将该菌株置于假诺卡氏科中，与 Pseudonocardia xishanensis JCM 17906（98.8%）、Pseudonocardia oroxyli DSM 44984（98.7%）、Pseudonocardia thailandensis CMU-NKS-70（98.7%）和 Pseudonocardia ailaonensis DSM 44979（97.9%）最为密切相关。多相研究的结果包含 ANIb、ANI m 和数字 DNA-DNA 杂交的基因组比较，表明菌株 CAP47R 与具有有效名称的最接近物种存在分化。该菌株代表了一个新物种，提议的这个微生物的名称为松发假诺卡氏菌（Pseudonocardia pini）sp. nov.，表示该放线菌来源于松树。模式菌株为 CAP47R（= DSM 108967 = NRRL B-65534）。基因组挖掘表明，该菌株含有多种编码可降解有害化学物质的酶的基因。
